Characterization of M1-8-positive cells in the peripheral blood and bone marrow of mice

A presumption on the origin of dendritic cells

S. Tanaka, T. Krenács, H. Uda, H. Sakamoto

Research output: Contribution to journalArticle

Abstract

It has previously been shown that monoclonal antibody M1-8 can recognize murine dendritic cells (DC) in the skin and T-dependent area of lymphoid organs. Recently we found some peripheral blood mononuclear cells (PBMC) and bone marrow cells (BMC) that also express M1-8 antigen. In order to get closer to the origin of DC, we characterized these cells in this study. Flow cytometry showed that 1%-4% of PBMC and 15%-30% of BMC were positive for M1-8 antigen. Most of M1-8-positive BMC also expressed ER-MP 20 antigen, a marker of macrophage precursors, although no mature monocytes in the peripheral blood reacted with the M1-8 antibody. Among the M1- 8-positive cells, a higher percentage of PBMC than BMC expressed Thy 1.2 and 1a antigen, while M1-8-reactive BMC more frequently expressed Fc receptors than PBMC. Therefore, more M1-8-positive BMC showed monocytoid characters than PBMC. Ultrastructurally, M1-8 antibody reacted to the cytoplasm of the lymphocyte-like cells both in the bone marrow and peripheral blood. In addition, some cells with monoblastoid/cytoid morphology and immature peroxidase-positive granules were also M1-8-positive in the bone marrow. Our results suggest that M1-8-positive PBMC and BMC could be immature cells of monoblastoid/cytoid origin, distinct from classic monocytes, and they may serve as precursors for peripheral DC.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalCell Vision - Journal of Analytical Morphology
Volume3
Issue number2
Publication statusPublished - 1996

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Bone Marrow Cells
Dendritic Cells
Bone Marrow
Blood Cells
Monocytes
Thy-1 Antigens
Antigens
Langerhans Cells
Fc Receptors
Antibodies
Differentiation Antigens
Peroxidase
Flow Cytometry
Cytoplasm
Macrophages
Monoclonal Antibodies
Lymphocytes

ASJC Scopus subject areas

  • Anatomy

Cite this

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title = "Characterization of M1-8-positive cells in the peripheral blood and bone marrow of mice: A presumption on the origin of dendritic cells",
abstract = "It has previously been shown that monoclonal antibody M1-8 can recognize murine dendritic cells (DC) in the skin and T-dependent area of lymphoid organs. Recently we found some peripheral blood mononuclear cells (PBMC) and bone marrow cells (BMC) that also express M1-8 antigen. In order to get closer to the origin of DC, we characterized these cells in this study. Flow cytometry showed that 1{\%}-4{\%} of PBMC and 15{\%}-30{\%} of BMC were positive for M1-8 antigen. Most of M1-8-positive BMC also expressed ER-MP 20 antigen, a marker of macrophage precursors, although no mature monocytes in the peripheral blood reacted with the M1-8 antibody. Among the M1- 8-positive cells, a higher percentage of PBMC than BMC expressed Thy 1.2 and 1a antigen, while M1-8-reactive BMC more frequently expressed Fc receptors than PBMC. Therefore, more M1-8-positive BMC showed monocytoid characters than PBMC. Ultrastructurally, M1-8 antibody reacted to the cytoplasm of the lymphocyte-like cells both in the bone marrow and peripheral blood. In addition, some cells with monoblastoid/cytoid morphology and immature peroxidase-positive granules were also M1-8-positive in the bone marrow. Our results suggest that M1-8-positive PBMC and BMC could be immature cells of monoblastoid/cytoid origin, distinct from classic monocytes, and they may serve as precursors for peripheral DC.",
author = "S. Tanaka and T. Kren{\'a}cs and H. Uda and H. Sakamoto",
year = "1996",
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T1 - Characterization of M1-8-positive cells in the peripheral blood and bone marrow of mice

T2 - A presumption on the origin of dendritic cells

AU - Tanaka, S.

AU - Krenács, T.

AU - Uda, H.

AU - Sakamoto, H.

PY - 1996

Y1 - 1996

N2 - It has previously been shown that monoclonal antibody M1-8 can recognize murine dendritic cells (DC) in the skin and T-dependent area of lymphoid organs. Recently we found some peripheral blood mononuclear cells (PBMC) and bone marrow cells (BMC) that also express M1-8 antigen. In order to get closer to the origin of DC, we characterized these cells in this study. Flow cytometry showed that 1%-4% of PBMC and 15%-30% of BMC were positive for M1-8 antigen. Most of M1-8-positive BMC also expressed ER-MP 20 antigen, a marker of macrophage precursors, although no mature monocytes in the peripheral blood reacted with the M1-8 antibody. Among the M1- 8-positive cells, a higher percentage of PBMC than BMC expressed Thy 1.2 and 1a antigen, while M1-8-reactive BMC more frequently expressed Fc receptors than PBMC. Therefore, more M1-8-positive BMC showed monocytoid characters than PBMC. Ultrastructurally, M1-8 antibody reacted to the cytoplasm of the lymphocyte-like cells both in the bone marrow and peripheral blood. In addition, some cells with monoblastoid/cytoid morphology and immature peroxidase-positive granules were also M1-8-positive in the bone marrow. Our results suggest that M1-8-positive PBMC and BMC could be immature cells of monoblastoid/cytoid origin, distinct from classic monocytes, and they may serve as precursors for peripheral DC.

AB - It has previously been shown that monoclonal antibody M1-8 can recognize murine dendritic cells (DC) in the skin and T-dependent area of lymphoid organs. Recently we found some peripheral blood mononuclear cells (PBMC) and bone marrow cells (BMC) that also express M1-8 antigen. In order to get closer to the origin of DC, we characterized these cells in this study. Flow cytometry showed that 1%-4% of PBMC and 15%-30% of BMC were positive for M1-8 antigen. Most of M1-8-positive BMC also expressed ER-MP 20 antigen, a marker of macrophage precursors, although no mature monocytes in the peripheral blood reacted with the M1-8 antibody. Among the M1- 8-positive cells, a higher percentage of PBMC than BMC expressed Thy 1.2 and 1a antigen, while M1-8-reactive BMC more frequently expressed Fc receptors than PBMC. Therefore, more M1-8-positive BMC showed monocytoid characters than PBMC. Ultrastructurally, M1-8 antibody reacted to the cytoplasm of the lymphocyte-like cells both in the bone marrow and peripheral blood. In addition, some cells with monoblastoid/cytoid morphology and immature peroxidase-positive granules were also M1-8-positive in the bone marrow. Our results suggest that M1-8-positive PBMC and BMC could be immature cells of monoblastoid/cytoid origin, distinct from classic monocytes, and they may serve as precursors for peripheral DC.

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