Characterization of liver-specific expression of rat uricase using monoclonal antibodies and cloned cDNAs

Kiyoto Motojima, S. Goto

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Tissue distribution of uricase (urate oxidase, EC 1.7.3.3) was studied by immunoblotting and RNA slot blot analysis. For immunoblotting, highly specific monoclonal antibodies against rat liver uricase were obtained, and for mRNA detection, a cloned uricase cDNA was used. Among seven tissues studied, uricase was immunologically detected only in the liver. The contents of uricase in other tissues, i.e., brain, thymus, heart, spleen, kidney and lactating mammary gland, were estimated to be less than 2% of that in the liver. Uricase mRNA was also detected only in the liver. The steady-state level of the mRNA in the isolated hepatocytes was relatively constant during the 8-day culture period when compared with those of other mRNAs expressed in the liver, suggesting a unique control mechanism of its expression.

Original languageEnglish
Pages (from-to)316-322
Number of pages7
JournalBBA - Gene Structure and Expression
Volume1087
Issue number3
DOIs
Publication statusPublished - Nov 30 1990

Fingerprint

Urate Oxidase
Liver
Rats
Complementary DNA
Monoclonal Antibodies
Messenger RNA
Tissue
Immunoblotting
Thymus
Tissue Distribution
Human Mammary Glands
Thymus Gland
Hepatocytes
Brain
Spleen
RNA
Kidney

Keywords

  • (Hepatocyte)
  • (Rat liver)
  • Liver-specific gene expression
  • Monoclonal antibody
  • Peroxisome
  • Uricase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Characterization of liver-specific expression of rat uricase using monoclonal antibodies and cloned cDNAs. / Motojima, Kiyoto; Goto, S.

In: BBA - Gene Structure and Expression, Vol. 1087, No. 3, 30.11.1990, p. 316-322.

Research output: Contribution to journalArticle

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