Characterization of intrinsic efflux activity of enterococcus faecalis ATCC29212 by a semi-automated ethidium bromide method

G. Spengler, Ana Martins, Zsuzsnna Schelz, Liliana Rodrigues, Lone Aagaard, Marta Martins, Sofia Santos Costa, Isabel Couto, Miguel Vivieros, Séamus Fanning, Jette E. Kristiansen, J. Molnár, Leonard Amaral

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Enterococcus faecalis is recognized as a multidrug- resistant nosocomial pathogen. The phenotypic basis for this is largely uncharacterized. The intrinsic efflux system of the antibiotic-susceptible E. faecalis ATCC29212 strain was studied using a semi-automated method that assesses accumulation and efflux of the universal efflux pump substrate ethidium bromide (EB). The results show that the intrinsic efflux system of this Enterococcus strain is controlled by energy derived from the catabolism of glucose and the proton concentration of the medium. At pH 5, agents that inhibit efflux pumps in Gram-positive organisms and the proton gradient un-coupler CCCP do not increase accumulation nor inhibit efflux of EB. In contrast, at pH 8, where the proton concentration is 1,000-fold lower, these agents increase accumulation and efflux of EB. These results are relevant to infections produced by E. faecalis and subsequent antibiotic therapy with antibiotics to which the organism is known to be intrinsically resistant.

Original languageEnglish
Pages (from-to)81-88
Number of pages8
JournalIn Vivo
Volume23
Issue number1
Publication statusPublished - Jan 2009

Fingerprint

Ethidium
Enterococcus faecalis
Protons
Anti-Bacterial Agents
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Pumps
Enterococcus
Pathogens
Glucose
Substrates
Infection
Therapeutics

Keywords

  • ABC transporters
  • Efflux pump inhibitors (EPIs)
  • PH
  • Proton motive force
  • Semi-automated EB method

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Spengler, G., Martins, A., Schelz, Z., Rodrigues, L., Aagaard, L., Martins, M., ... Amaral, L. (2009). Characterization of intrinsic efflux activity of enterococcus faecalis ATCC29212 by a semi-automated ethidium bromide method. In Vivo, 23(1), 81-88.

Characterization of intrinsic efflux activity of enterococcus faecalis ATCC29212 by a semi-automated ethidium bromide method. / Spengler, G.; Martins, Ana; Schelz, Zsuzsnna; Rodrigues, Liliana; Aagaard, Lone; Martins, Marta; Santos Costa, Sofia; Couto, Isabel; Vivieros, Miguel; Fanning, Séamus; Kristiansen, Jette E.; Molnár, J.; Amaral, Leonard.

In: In Vivo, Vol. 23, No. 1, 01.2009, p. 81-88.

Research output: Contribution to journalArticle

Spengler, G, Martins, A, Schelz, Z, Rodrigues, L, Aagaard, L, Martins, M, Santos Costa, S, Couto, I, Vivieros, M, Fanning, S, Kristiansen, JE, Molnár, J & Amaral, L 2009, 'Characterization of intrinsic efflux activity of enterococcus faecalis ATCC29212 by a semi-automated ethidium bromide method', In Vivo, vol. 23, no. 1, pp. 81-88.
Spengler, G. ; Martins, Ana ; Schelz, Zsuzsnna ; Rodrigues, Liliana ; Aagaard, Lone ; Martins, Marta ; Santos Costa, Sofia ; Couto, Isabel ; Vivieros, Miguel ; Fanning, Séamus ; Kristiansen, Jette E. ; Molnár, J. ; Amaral, Leonard. / Characterization of intrinsic efflux activity of enterococcus faecalis ATCC29212 by a semi-automated ethidium bromide method. In: In Vivo. 2009 ; Vol. 23, No. 1. pp. 81-88.
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