Characterization of intestinal cnf1+ Escherichia coli from weaned pigs

Istvan Tóth, Eric Oswald, Jacques G. Mainil, Mohamed Awad-Masalmeh, Béla Nagy

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Escherichia coli isolated from 204 cases of porcine postweaning diarrhoea were tested by PCR for the genes of cytotoxic necrotic factors (CNF) and of cytolethal dystending toxin (CDT). Selected strains were also examined by PCR for the presence of papC-, sfa-, f17-, f18-, and afa-specific sequences encoding P, S, F17, F18 fimbriae and afimbrial adhesins. A 5.9% (12/204) of the strains had cnf1 gene, and two of them had cdt gene as well. Further six cdt+ strains were detected which were cnf-negative. Most of the cnf1+ strains belonged to serogroups O2, O6, O8, O54 characteristic of necrotoxic E. coli (NTEC) of humans. All the cnf1+ strains possessed the genes for P or S fimbriae or both, but were negative for F4, F17, or F18 or afimbrial adhesins. Results suggest that these enteric isolates may have entero- and/or uropathogenic significance in weaned pigs, and may have zoonotic potential for humans.

Original languageEnglish
Pages (from-to)539-542
Number of pages4
JournalInternational Journal of Medical Microbiology
Volume290
Issue number6
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • CDT
  • CNF
  • Escherichia coli
  • PapC
  • Pig
  • Sfa

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)
  • Infectious Diseases

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