Characterization of human invariant natural killer T cells expressing foxP3

P. Engelmann, Klára Farkas, János Kis, Geoffrey Richman, Zhaoyun Zhang, Chong Wee Liew, Maciej Borowiec, Monika A. Niewczas, Heyam Jalahej, Tihamér Orbán

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Recently described forkhead box protein 3 (FoxP3) transcription factor is a key molecule in CD4+ CD25hi+ T-cell characterization. Invariant NK T (iNKT) cells are also characterized as regulatory cells modulating the immune response by rapidly producing T h1 and T h2 cytokines. We aimed to analyze cellular markers important in regulatory features of human iNKT cells and to study their role in functional assays. iNKT cells were single cell sorted from peripheral mononuclear cells of healthy individuals after immunostaining of invariant TCR α-chain. We found FoxP3 expression in human iNKT clones. Randomly selected iNKT cell clones (CD4+, double negative, CD8+) expressed FoxP3 mRNA and protein at different levels upon stimulation as supported by various approaches. FoxP3 mRNA and protein expression was detected in unstimulated iNKT cells as well. Furthermore, different stimulations changed the FoxP3 expression in iNKT cells over time and the most dramatic changes were observed upon anti-CD3 stimulation. Both the supernatant of iNKT cells and iNKT cells themselves exerted similar stimulation effects on PBMC proliferation in functional assays and these stimulations showed a negative correlation with FoxP3 expression. Our data indicate that the FoxP3 expression in iNKT cells may be a key transcriptional factor in controlling the regulatory function of the iNKT cells.

Original languageEnglish
Pages (from-to)473-484
Number of pages12
JournalInternational Immunology
Volume23
Issue number8
DOIs
Publication statusPublished - 2011

Fingerprint

Natural Killer T-Cells
Natural Killer Cells
Forkhead Transcription Factors
T-Lymphocytes
Clone Cells
Transcription Factor 3
CD4 Antigens
Messenger RNA
Proteins
Cytokines

Keywords

  • Cytokines
  • Human iNKT cells
  • Immune regulation
  • Regulatory T cells

ASJC Scopus subject areas

  • Immunology

Cite this

Engelmann, P., Farkas, K., Kis, J., Richman, G., Zhang, Z., Liew, C. W., ... Orbán, T. (2011). Characterization of human invariant natural killer T cells expressing foxP3. International Immunology, 23(8), 473-484. https://doi.org/10.1093/intimm/dxr040

Characterization of human invariant natural killer T cells expressing foxP3. / Engelmann, P.; Farkas, Klára; Kis, János; Richman, Geoffrey; Zhang, Zhaoyun; Liew, Chong Wee; Borowiec, Maciej; Niewczas, Monika A.; Jalahej, Heyam; Orbán, Tihamér.

In: International Immunology, Vol. 23, No. 8, 2011, p. 473-484.

Research output: Contribution to journalArticle

Engelmann, P, Farkas, K, Kis, J, Richman, G, Zhang, Z, Liew, CW, Borowiec, M, Niewczas, MA, Jalahej, H & Orbán, T 2011, 'Characterization of human invariant natural killer T cells expressing foxP3', International Immunology, vol. 23, no. 8, pp. 473-484. https://doi.org/10.1093/intimm/dxr040
Engelmann, P. ; Farkas, Klára ; Kis, János ; Richman, Geoffrey ; Zhang, Zhaoyun ; Liew, Chong Wee ; Borowiec, Maciej ; Niewczas, Monika A. ; Jalahej, Heyam ; Orbán, Tihamér. / Characterization of human invariant natural killer T cells expressing foxP3. In: International Immunology. 2011 ; Vol. 23, No. 8. pp. 473-484.
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