Characterization of high-affinity 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE) binding sites on normal human keratinocytes

P. Arenberger, L. Kemény, T. Ruzicka

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Eicosanoids are thought to play an important role in the pathogenesis of inflammatory skin diseases. The object of the present study was the detection and characterization of putative 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE) binding sites in normal human keratinocytes. Keratinocytes were obtained from foreskin and dermatome-shaved normal human skin. Radioligand binding assays were performed with 12(S)[3H]HETE on cultured cells. Analysis of saturation curves suggested a one-site model for 12(S)-HETE binding with a K(D) Of 3.84 ± 0.18 nM and receptor number B(max) of 2.32 ± 0.12 x 105 per cell. Ligand binding was reversible. The rank order of potency in competition for 12(S)-[3H]HETE was 12(S)HETE>12(R)-HETE≥leukotriene B4. Preincubation of cells with 12(S)-HETE (2Xl0-6M) resulted in down-regulation of the binding site by approximately 50%. The identification and characterization of specific 12(S)HETE binding sites on normal human keratinocytes should enable further elucidation of the role of 12-HETE in cutaneous biology and in the pathophysiology of psoriasis and other inflammatory and hyperproliferative dermatoses.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalEpithelial Cell Biology
Volume2
Issue number1
Publication statusPublished - 1993

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12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Keratinocytes
Binding Sites
Skin Diseases
Foreskin
Radioligand Assay
Skin
Eicosanoids
Psoriasis
Cultured Cells
Down-Regulation
Ligands

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology

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Characterization of high-affinity 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE) binding sites on normal human keratinocytes. / Arenberger, P.; Kemény, L.; Ruzicka, T.

In: Epithelial Cell Biology, Vol. 2, No. 1, 1993, p. 1-6.

Research output: Contribution to journalArticle

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