Characterization of francisella tularensis strains, comparing their carbon source utilization

M. Gyuranecz, K. Erdélyi, L. Fodor, K. Jánosi, B. Szépe, M. Füleki, I. Szke, B. Dénes, L. Makrai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Thirteen Francisella tularensis strains were isolated from 22 seropositive brown hares (Lepus europaeus) originating from different parts of Hungary, and further two from a patas monkey (Erythrocebus patas) and vervet monkey (Chlorocebus aethiops). The isolates were identified as F. tularensis ssp. holarctica on the basis of culture, morphological and biochemical characteristics. The identification was verified by polymerase chain reaction and the sequencing of the partial 16S rRNA gene. Utilization of carbon sources of the 15 F. tularensis strains was characterized with the Biolog system. The system was able to identify the strains already after 4 h of incubation, not only after the standard 24 h. After the analysis and comparison of the metabolic profiles of our strains with the Biolog database, it was concluded that not all carbon sources indicated in the database were utilized by our isolates. The Biolog software fails to distinguish the highly virulent F. tularensis ssp. tularensis and the moderately virulent F. tularensis ssp. holarctica but the Biolog microplates can be manually read to differentiate the two subspecies based on glycerol source utilization. As all the studied strains were unable to use glycerol, they could be identified as F. tularensis ssp. holarctica. The dendrogram based on the metabolic relationship of the strains shows that the isolates are very similar to each other, which correlates with the conservative genetic character of F. tularensis ssp. holarctica.

Original languageEnglish
Pages (from-to)417-422
Number of pages6
JournalZoonoses and Public Health
Volume57
Issue number6
DOIs
Publication statusPublished - Sep 2010

Fingerprint

Francisella tularensis
Carbon
Erythrocebus patas
carbon
Hares
Cercopithecus aethiops
Glycerol
glycerol
Erythrocebus
Databases
Lepus
Metabolome
Hungary
hares
rRNA Genes
monkeys
Software
polymerase chain reaction
ribosomal RNA
Polymerase Chain Reaction

Keywords

  • carbon source
  • Francisella tularensis
  • glycerol
  • tularemia
  • zoonosis

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Epidemiology
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Characterization of francisella tularensis strains, comparing their carbon source utilization. / Gyuranecz, M.; Erdélyi, K.; Fodor, L.; Jánosi, K.; Szépe, B.; Füleki, M.; Szke, I.; Dénes, B.; Makrai, L.

In: Zoonoses and Public Health, Vol. 57, No. 6, 09.2010, p. 417-422.

Research output: Contribution to journalArticle

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