Characterization of factor H-related cell membrane molecules expressed by human B lymphocytes and neutrophil granulocytes

Mihály Józsi, Attila Kapus, Krisztina Kerekes, József Kármán, Z. Bajtay, Peter F. Zipfel, A. Erdei

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The human factor H protein family comprises six plasma glycoproteins. Earlier we described a membranal factor H-related (mFHR) molecule that is expressed by human B lymphoblastoid cell lines and exerts cofactor activity. In our present study we screened human blood cells for the presence of mFHR proteins and further characterized these molecules. By cytofluorimetry it is shown that the factor H-specific rabbit antiserum reacts strongly with B cells and neutrophil granulocytes, but not with T cells and monocytes. On B lymphocytes mFHR is shown to be down-regulated upon activation of the cells via sIg. In experiments studying which short consensus repeat (SCR) domains are part of the cell membrane proteins we found that antibodies raised against SCRs 1-4, 19-20 and FHR-3 bound to neutrophils but not to B cells. While mFHRs derived both from B cells and granulocytes are shown to bind heparin, their size and structure are different as revealed by Western blotting. A further characteristic of the granulocyte-derived mFHR is its sensitivity to the PI-specific PLCγ enzyme. These data demonstrate the existence of new members of the FHR protein family, as two distinct, membranal forms are identified. Based on the differences, the B cell derived molecule is termed mFHR-1 and the neutrophil derived protein mFHR-2.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalImmunology Letters
Volume77
Issue number1
DOIs
Publication statusPublished - May 1 2001

Fingerprint

Complement Factor H
Granulocytes
Neutrophils
B-Lymphocytes
Cell Membrane
Proteins
Heparin
Immune Sera
Monocytes
Blood Cells
Glycoproteins
Membrane Proteins
Western Blotting
Rabbits
T-Lymphocytes
Cell Line
Antibodies
Enzymes

Keywords

  • B lymphocytes
  • Factor H-related
  • Heparin binding
  • Neutrophils

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Characterization of factor H-related cell membrane molecules expressed by human B lymphocytes and neutrophil granulocytes. / Józsi, Mihály; Kapus, Attila; Kerekes, Krisztina; Kármán, József; Bajtay, Z.; Zipfel, Peter F.; Erdei, A.

In: Immunology Letters, Vol. 77, No. 1, 01.05.2001, p. 55-62.

Research output: Contribution to journalArticle

Józsi, Mihály ; Kapus, Attila ; Kerekes, Krisztina ; Kármán, József ; Bajtay, Z. ; Zipfel, Peter F. ; Erdei, A. / Characterization of factor H-related cell membrane molecules expressed by human B lymphocytes and neutrophil granulocytes. In: Immunology Letters. 2001 ; Vol. 77, No. 1. pp. 55-62.
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