Characterization of extracellular γ-glutamyl transpeptidase from Aspergillus nidulans

Zsolt Spitzmüller, Sándor Gonda, Attila Kiss-Szikszai, G. Vasas, I. Pócsi, T. Emri

Research output: Contribution to journalArticle

Abstract

Aspergillus nidulans γ-glutamyl transpeptidase (AnγGT, EC 2.3.2.2) was partially purified from the fermentation broth of carbon stressed cultures. Its temperature and pH optimum was 45 °C and pH 8.0, respectively. AnγGT had little hydrolase activity. It utilized Gln, glutathione and less efficiently oxidized glutathione as γ-glutamyl donors (beside of γ-glutamyl-p-nitroanilide) and amino-acids and peptides (including Glu, Cys, Met, Gly-Gly and Cys-Gly) but not hydroxylamine as γ-glutamyl acceptors. We propose that the function of this enzyme is not to degrade, but to produce, γ-glutamyl compounds which may be related to the utilization of extracellular peptides and amino-acids in carbon stressed cultures.

Original languageEnglish
Pages (from-to)400-403
Number of pages4
JournalMycoscience
Volume57
Issue number6
DOIs
Publication statusPublished - Nov 1 2016

Fingerprint

Aspergillus nidulans
gamma-glutamyltransferase
peptide
glutathione
amino acid
peptides
amino acids
carbon
hydrolases
fermentation
enzyme
enzymes
temperature
hydroxylamine

Keywords

  • Carbon stress
  • Glutathione metabolism
  • Utilization of peptides
  • γ-Glutamyl transpeptidase substrates

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Characterization of extracellular γ-glutamyl transpeptidase from Aspergillus nidulans. / Spitzmüller, Zsolt; Gonda, Sándor; Kiss-Szikszai, Attila; Vasas, G.; Pócsi, I.; Emri, T.

In: Mycoscience, Vol. 57, No. 6, 01.11.2016, p. 400-403.

Research output: Contribution to journalArticle

Spitzmüller, Zsolt ; Gonda, Sándor ; Kiss-Szikszai, Attila ; Vasas, G. ; Pócsi, I. ; Emri, T. / Characterization of extracellular γ-glutamyl transpeptidase from Aspergillus nidulans. In: Mycoscience. 2016 ; Vol. 57, No. 6. pp. 400-403.
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