Characterization of excimer laser ablation generated pepsin particles using multi-wavelength photoacoustic instrument

B. Hopp, G. Kecskeméti, T. Smausz, T. Ajtai, A. Filep, N. Utry, A. Kohut, Z. Bozóki, G. Szabó

Research output: Contribution to journalArticle

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Abstract

Preparation of organic thin layers on various special substrates using the pulsed laser deposition (PLD) technique is an important task from the point of view of bioengineering and biosensor technologies. Earlier studies demonstrated that particle ejection starts during the ablating laser pulse resulting in significant shielding effects which can influence the real fluence on the target surface and consequently the efficiency of layer preparation. In this study, we introduce a photoacoustic absorption measurement technique for in-situ characterization of ablated particles during PLD experiments. A KrF excimer laser beam (λ = 248 nm, FWHM = 18 ns) was focused onto pepsin targets in a PLD chamber; the applied laser fluences were 440 and 660 mJ/cm 2. We determined the wavelength dependence of optical absorption and mass specific absorption coefficient of laser ablation generated pepsin aerosols in the UV-VIS- NIR range. On the basis of our measurements, we calculated the absorbance at the ablating laser wavelength, too. We demonstrated that when the laser ablation generated pepsin aerosols spread through the whole PLD chamber the effect of absorptivity is negligible for the subsequent pulses. However, the interaction of the laser pulse and the just formed particle cloud generated by the same pulse is more significant.

Original languageEnglish
Pages (from-to)429-435
Number of pages7
JournalApplied Physics A: Materials Science and Processing
Volume107
Issue number2
DOIs
Publication statusPublished - May 2012

Fingerprint

pepsin
Photoacoustic effect
Pepsin A
Excimer lasers
Laser ablation
Pulsed laser deposition
excimer lasers
laser ablation
pulsed laser deposition
Laser pulses
Wavelength
pulses
Aerosols
wavelengths
lasers
aerosols
absorptivity
fluence
chambers
bioengineering

ASJC Scopus subject areas

  • Materials Science(all)
  • Chemistry(all)

Cite this

Characterization of excimer laser ablation generated pepsin particles using multi-wavelength photoacoustic instrument. / Hopp, B.; Kecskeméti, G.; Smausz, T.; Ajtai, T.; Filep, A.; Utry, N.; Kohut, A.; Bozóki, Z.; Szabó, G.

In: Applied Physics A: Materials Science and Processing, Vol. 107, No. 2, 05.2012, p. 429-435.

Research output: Contribution to journalArticle

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