Characterization of cytokinins by electrospray ionization and collision-induced dissociation mass spectrometry without precursor ion selection

T. Bartók, G. Börcsök, R. Komoróczy, F. Sági

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Eight naturally occurring and four synthetic cytokinins were studied by means of electrospray ionization collision-induced dissociation single-quadrupole mass spectrometry in order to examine the potential of this technique in the mapping of their structures. At a low capillary exit voltage, protonated molecular ions dominated, but in the range 240-300 V informative and typical collision-induced fragmentation was obtained. At these voltages, protonated adenine and purine ions (m/z 136 and 119, respectively) were formed from all natural but not from the synthetic cytokinins, which produced other ions specific to the molecule. Nevertheless, the loss of 132 u ([M+H - C5H8O4]+) or 162 u ([M + H - C6H10O5]+) pointed to the presence of the ribosyl or glucosyl group in the two types of cytokinins. The occurrence of the molecular and 'diagnostic' ions, and the possible loss of 132 or 162 u, readily permit the characterization of the chemical structure and the identification of unknown cytokinin molecules.

Original languageEnglish
Pages (from-to)756-760
Number of pages5
JournalJournal of Mass Spectrometry
Volume31
Issue number7
DOIs
Publication statusPublished - Jul 1996

Fingerprint

Cytokinins
Electrospray ionization
Mass spectrometry
Mass Spectrometry
mass spectroscopy
dissociation
Ions
ionization
collisions
purines
ions
adenines
electric potential
molecular ions
Molecules
Molecular Pathology
molecules
fragmentation
Electric potential
Adenine

Keywords

  • Collision-induced dissociation
  • Cytokinins
  • Electrospray
  • Precursor ion selection

ASJC Scopus subject areas

  • Biophysics
  • Organic Chemistry
  • Spectroscopy

Cite this

Characterization of cytokinins by electrospray ionization and collision-induced dissociation mass spectrometry without precursor ion selection. / Bartók, T.; Börcsök, G.; Komoróczy, R.; Sági, F.

In: Journal of Mass Spectrometry, Vol. 31, No. 7, 07.1996, p. 756-760.

Research output: Contribution to journalArticle

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