Characterization of clinical vancomycin-resistant enterococcus faecium isolated in Eastern Hungary

Dorottya Franyó, Balázs Kocsi, Virág Lesinszki, Judit Pászti, Anita Kozák, Evelin Erzsébet Bukta, J. Szabó, Zsuzsanna Dombrádi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives: The aim of our study was to characterize and elicit the genetic relatedness of emerging vancomycin-resistant enterococci (VRE) isolated between 2012 and 2015 at a teaching hospital in Debrecen, Hungary. Results: Altogether 43 nonduplicate vancomycin-resistant Enterococcus faecium (VREfm) clinical isolates were obtained. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used for species identification. Isolates showed 100% resistance to ampicillin and ciprofloxacin while 81.4% were resistant to gentamicin. PCR analysis revealed the presence of VanB in 40 and VanA in 3 isolates. Among ace, agg, and esp virulence genes only esp was found in seven cases. Modified microtiter-plate test showed 13 weak and 4 moderate biofilm producer isolates. Pulsed-field gel electrophoresis revealed nine pulsotypes. According to multilocus sequence typing all of the tested isolates belonged to clonal complex 17 (CC17). Conclusions: We report on the alarming emergence of multidrug-resistant VREfm belonging to CC17 at a tertiary hospital in Eastern Hungary. This is the first report of sequence types 412 and 364 from this region. Although outbreak did not occur the increasing prevalence of VREfm is of concern and dissemination must be prevented with proper infection control measures and regular VRE screening.

Original languageEnglish
Pages (from-to)1559-1567
Number of pages9
JournalMicrobial Drug Resistance
Volume24
Issue number10
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Enterococcus faecium
Hungary
Ampicillin Resistance
Multilocus Sequence Typing
Pulsed Field Gel Electrophoresis
Ciprofloxacin
Biofilms
Infection Control
Gentamicins
Tertiary Care Centers
Teaching Hospitals
Disease Outbreaks
Virulence
Mass Spectrometry
Lasers
Vancomycin-Resistant Enterococci
Polymerase Chain Reaction
Genes

Keywords

  • Biofilm
  • Esp
  • MALDI-TOF
  • Multilocus sequence typing
  • PFGE
  • VREfm

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Pharmacology
  • Microbiology (medical)

Cite this

Franyó, D., Kocsi, B., Lesinszki, V., Pászti, J., Kozák, A., Bukta, E. E., ... Dombrádi, Z. (2018). Characterization of clinical vancomycin-resistant enterococcus faecium isolated in Eastern Hungary. Microbial Drug Resistance, 24(10), 1559-1567. https://doi.org/10.1089/mdr.2018.0074

Characterization of clinical vancomycin-resistant enterococcus faecium isolated in Eastern Hungary. / Franyó, Dorottya; Kocsi, Balázs; Lesinszki, Virág; Pászti, Judit; Kozák, Anita; Bukta, Evelin Erzsébet; Szabó, J.; Dombrádi, Zsuzsanna.

In: Microbial Drug Resistance, Vol. 24, No. 10, 01.12.2018, p. 1559-1567.

Research output: Contribution to journalArticle

Franyó, D, Kocsi, B, Lesinszki, V, Pászti, J, Kozák, A, Bukta, EE, Szabó, J & Dombrádi, Z 2018, 'Characterization of clinical vancomycin-resistant enterococcus faecium isolated in Eastern Hungary', Microbial Drug Resistance, vol. 24, no. 10, pp. 1559-1567. https://doi.org/10.1089/mdr.2018.0074
Franyó, Dorottya ; Kocsi, Balázs ; Lesinszki, Virág ; Pászti, Judit ; Kozák, Anita ; Bukta, Evelin Erzsébet ; Szabó, J. ; Dombrádi, Zsuzsanna. / Characterization of clinical vancomycin-resistant enterococcus faecium isolated in Eastern Hungary. In: Microbial Drug Resistance. 2018 ; Vol. 24, No. 10. pp. 1559-1567.
@article{87c98d8acfcf485492e95998342bacd4,
title = "Characterization of clinical vancomycin-resistant enterococcus faecium isolated in Eastern Hungary",
abstract = "Objectives: The aim of our study was to characterize and elicit the genetic relatedness of emerging vancomycin-resistant enterococci (VRE) isolated between 2012 and 2015 at a teaching hospital in Debrecen, Hungary. Results: Altogether 43 nonduplicate vancomycin-resistant Enterococcus faecium (VREfm) clinical isolates were obtained. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used for species identification. Isolates showed 100{\%} resistance to ampicillin and ciprofloxacin while 81.4{\%} were resistant to gentamicin. PCR analysis revealed the presence of VanB in 40 and VanA in 3 isolates. Among ace, agg, and esp virulence genes only esp was found in seven cases. Modified microtiter-plate test showed 13 weak and 4 moderate biofilm producer isolates. Pulsed-field gel electrophoresis revealed nine pulsotypes. According to multilocus sequence typing all of the tested isolates belonged to clonal complex 17 (CC17). Conclusions: We report on the alarming emergence of multidrug-resistant VREfm belonging to CC17 at a tertiary hospital in Eastern Hungary. This is the first report of sequence types 412 and 364 from this region. Although outbreak did not occur the increasing prevalence of VREfm is of concern and dissemination must be prevented with proper infection control measures and regular VRE screening.",
keywords = "Biofilm, Esp, MALDI-TOF, Multilocus sequence typing, PFGE, VREfm",
author = "Dorottya Frany{\'o} and Bal{\'a}zs Kocsi and Vir{\'a}g Lesinszki and Judit P{\'a}szti and Anita Koz{\'a}k and Bukta, {Evelin Erzs{\'e}bet} and J. Szab{\'o} and Zsuzsanna Dombr{\'a}di",
year = "2018",
month = "12",
day = "1",
doi = "10.1089/mdr.2018.0074",
language = "English",
volume = "24",
pages = "1559--1567",
journal = "Microbial Drug Resistance",
issn = "1076-6294",
publisher = "Mary Ann Liebert Inc.",
number = "10",

}

TY - JOUR

T1 - Characterization of clinical vancomycin-resistant enterococcus faecium isolated in Eastern Hungary

AU - Franyó, Dorottya

AU - Kocsi, Balázs

AU - Lesinszki, Virág

AU - Pászti, Judit

AU - Kozák, Anita

AU - Bukta, Evelin Erzsébet

AU - Szabó, J.

AU - Dombrádi, Zsuzsanna

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Objectives: The aim of our study was to characterize and elicit the genetic relatedness of emerging vancomycin-resistant enterococci (VRE) isolated between 2012 and 2015 at a teaching hospital in Debrecen, Hungary. Results: Altogether 43 nonduplicate vancomycin-resistant Enterococcus faecium (VREfm) clinical isolates were obtained. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used for species identification. Isolates showed 100% resistance to ampicillin and ciprofloxacin while 81.4% were resistant to gentamicin. PCR analysis revealed the presence of VanB in 40 and VanA in 3 isolates. Among ace, agg, and esp virulence genes only esp was found in seven cases. Modified microtiter-plate test showed 13 weak and 4 moderate biofilm producer isolates. Pulsed-field gel electrophoresis revealed nine pulsotypes. According to multilocus sequence typing all of the tested isolates belonged to clonal complex 17 (CC17). Conclusions: We report on the alarming emergence of multidrug-resistant VREfm belonging to CC17 at a tertiary hospital in Eastern Hungary. This is the first report of sequence types 412 and 364 from this region. Although outbreak did not occur the increasing prevalence of VREfm is of concern and dissemination must be prevented with proper infection control measures and regular VRE screening.

AB - Objectives: The aim of our study was to characterize and elicit the genetic relatedness of emerging vancomycin-resistant enterococci (VRE) isolated between 2012 and 2015 at a teaching hospital in Debrecen, Hungary. Results: Altogether 43 nonduplicate vancomycin-resistant Enterococcus faecium (VREfm) clinical isolates were obtained. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used for species identification. Isolates showed 100% resistance to ampicillin and ciprofloxacin while 81.4% were resistant to gentamicin. PCR analysis revealed the presence of VanB in 40 and VanA in 3 isolates. Among ace, agg, and esp virulence genes only esp was found in seven cases. Modified microtiter-plate test showed 13 weak and 4 moderate biofilm producer isolates. Pulsed-field gel electrophoresis revealed nine pulsotypes. According to multilocus sequence typing all of the tested isolates belonged to clonal complex 17 (CC17). Conclusions: We report on the alarming emergence of multidrug-resistant VREfm belonging to CC17 at a tertiary hospital in Eastern Hungary. This is the first report of sequence types 412 and 364 from this region. Although outbreak did not occur the increasing prevalence of VREfm is of concern and dissemination must be prevented with proper infection control measures and regular VRE screening.

KW - Biofilm

KW - Esp

KW - MALDI-TOF

KW - Multilocus sequence typing

KW - PFGE

KW - VREfm

UR - http://www.scopus.com/inward/record.url?scp=85058414986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85058414986&partnerID=8YFLogxK

U2 - 10.1089/mdr.2018.0074

DO - 10.1089/mdr.2018.0074

M3 - Article

AN - SCOPUS:85058414986

VL - 24

SP - 1559

EP - 1567

JO - Microbial Drug Resistance

JF - Microbial Drug Resistance

SN - 1076-6294

IS - 10

ER -