Characterization of chromosome-specific S-SAP markers and their use in studying genetic diversity in Aegilops species

Ervin D. Nagy, István Molnár, Annamária Schneider, G. Kovács, M. Molnár-Láng

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The short interspersed nuclear element (SINE), Au, was used to develop sequence-specific amplified polymorphism (S-SAP) markers for U- and M-genome chromosomes. The markers were localized using Triticum aestivum (wheat) - Aegilops geniculata and wheat - Aegilops biuncialis disomic chromosome addition lines. Thirty-seven markers distributed over 6 U and 6 M chromosomes were produced. A genetic diversity study carried out on 37 accessions from Ae. biuncialis, Ae. comosa, Ae. geniculata, and Ae. umbellulata suggested that Ae. biuncialis have arisen from its diploid ancestors more recently than Ae. geniculata. Several earlier studies indicated that the M genomes in polyploid Aegilops species had accumulated substantial rearrangements, whereas the U genomes remained essentially unmodified. However, this cannot be attributed to the preferential insertion of retroelements into the M genome chromosomes. Fourteen markers from a total of 8 chromosomes were sequenced; 3 markers were similar to known plant genes, 1 was derived from a long terminal repeat (LTR) retrotransposon, and 10 markers did not match to any known DNA sequences, suggesting that they were located in the highly variable intergenic regions.

Original languageEnglish
Pages (from-to)289-296
Number of pages8
JournalGenome
Volume49
Issue number4
DOIs
Publication statusPublished - Apr 2006

Fingerprint

Aegilops
Aegilops biuncialis
Aegilops geniculata
Chromosomes
genetic polymorphism
Genome
Triticum
chromosomes
Retroelements
genetic variation
genome
retrotransposons
chromosome addition
disomics
Plant Genes
Chromosomes, Human, Pair 8
Chromosomes, Human, Pair 6
wheat
Intergenic DNA
Polyploidy

Keywords

  • Aegilops
  • Genetic diversity
  • S-SAP
  • Sequence characterization
  • U and M genomes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biotechnology
  • Genetics
  • Genetics(clinical)

Cite this

Characterization of chromosome-specific S-SAP markers and their use in studying genetic diversity in Aegilops species. / Nagy, Ervin D.; Molnár, István; Schneider, Annamária; Kovács, G.; Molnár-Láng, M.

In: Genome, Vol. 49, No. 4, 04.2006, p. 289-296.

Research output: Contribution to journalArticle

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