Characterization of chromate-sensitive and -tolerant mutants of Schizosaccharomyces pombe

K. Czakó-Vér, Z. Koósz, J. Antal, T. Rácz, M. Sipiczki, M. Pesti

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3 Citations (Scopus)

Abstract

Stable chromium(VI)-sensitive and -tolerant mutants were obtained by induced mutagenesis of Schizosaccharomyces pombe lysine and leucine auxotrophic heterothallic strains 6chr+ and 9chr+. Eleven of them were selected for further studies. Fast transport of 51CrO 42- was detected in a representative sensitive mutant, chr-51S, while the tolerant mutant chr1-66T and the parental strain 6chr + exhibited significantly lower 51CrO4 2- uptake. The segregation of tetrads of three selected Cr VI-tolerant mutants, chr1-66T, chr1-14T and chr2-04T, strongly indicated that tolerance was determined by single mutations. Random spore analysis proved that the mutations of chr1-66T and chr1-14T were allelic and the mutation of mutant chr2-04T was not allelic with the mutation of chr1-66T. Recombinants carrying the ura4D18 selective marker were created for transformation experiments. Two of them (chr1-661T and chr2-046T) can be used to clone and identify the genes responsible for their CrVI tolerance phenotype.

Original languageEnglish
Pages (from-to)31-36
Number of pages6
JournalFolia Microbiologica
Volume49
Issue number1
DOIs
Publication statusPublished - Jan 1 2004

ASJC Scopus subject areas

  • Microbiology

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