Characterization of canine pericardial fluid endothelin-1 levels

I. Szokodi, F. Horkay, Pál Kiss, L. Selmeci, I. Horváth, Olli Vuolteenaho, Heikki Ruskoaho, A. Juhász-Nagy, Miklós Tóth

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Recently, extremely high concentrations of endothelin-1 (ET-1) were detected in the pericardial fluid of patients undergoing cardiac surgery. This study was designed to characterize ET-1 levels in plasma and pericardial fluid of dogs. Plasma and pericardial fluid samples were extracted using SepPak C18 cartridges and ET-1 levels were measured by a specific radioimmunoassay. Pericardial fluid ET-1 levels (739 ± 81 pg/ml; n = 10) were significantly higher than respective plasma levels (22.4 ± 4.0 pg/ml; p <0.05). The mean pericardial fluid: plasma ratio of ir-ET-1 concentration was 54.7 ± 16.8. In HPLC analysis, the total ET-1-like immunoreactivity of pericardial fluid co-eluted with ET-1 standard. In anesthetized dogs (n = 6), disappearance of [125I]ET-1 was sixfold slower in the pericardial space than in plasma. Our results show that high concentrations of ir-ET-1 can be found in canine pericardial fluid. Slow elimination of ET-1 from the pericardial fluid compartment may contribute to high peptide levels.

Original languageEnglish
JournalJournal of Cardiovascular Pharmacology
Volume31
Issue numberSUPPL. 1
Publication statusPublished - 1998

Fingerprint

Endothelin-1
Canidae
Pericardial Fluid
Dogs
Pericardium
Thoracic Surgery
Radioimmunoassay
High Pressure Liquid Chromatography
Peptides

Keywords

  • Elimination
  • Endothelin-1
  • Pericardial fluid

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

Cite this

Characterization of canine pericardial fluid endothelin-1 levels. / Szokodi, I.; Horkay, F.; Kiss, Pál; Selmeci, L.; Horváth, I.; Vuolteenaho, Olli; Ruskoaho, Heikki; Juhász-Nagy, A.; Tóth, Miklós.

In: Journal of Cardiovascular Pharmacology, Vol. 31, No. SUPPL. 1, 1998.

Research output: Contribution to journalArticle

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AU - Horkay, F.

AU - Kiss, Pál

AU - Selmeci, L.

AU - Horváth, I.

AU - Vuolteenaho, Olli

AU - Ruskoaho, Heikki

AU - Juhász-Nagy, A.

AU - Tóth, Miklós

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