Characterization of Candida albicans colony-morphology mutants and their hybrids

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The collection wild-type strain of Candida albicans was used to obtain auxotrophic and colony-morphology mutants by 'nitrosoguanidine' treatment. Intraspecific protoplast fusion induced by Ca2+-poly(ethyleneglycol) was carried out in various pairings between the auxotrophic strain producing smooth colonies and containing blastospores and the colony-morphology mutants containing a mixture of blastospores and pseudohyphae or only hyphae. Hybrids exhibiting full or partial complementation were obtained when mutants producing smooth colonies and colony-morphology variants of different origins were fused. The mutation responsible for the colony-morphology character (if various types of colony morphomutants were crossed) proved to be recessive or semidominant. Representative hybrids exhibited elevated DNA contents as measured by flow cytometry. To illustrate various cell types, and especially the intermediate one (never observed in natural isolates), a preparation method was further developed for scanning electron microscopic studies.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalFolia Microbiologica
Volume48
Issue number2
DOIs
Publication statusPublished - Mar 2003

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Candida albicans
Nitrosoguanidines
Hyphae
Protoplasts
Flow Cytometry
Electrons
Mutation
DNA

ASJC Scopus subject areas

  • Microbiology

Cite this

Characterization of Candida albicans colony-morphology mutants and their hybrids. / Novák, A.; Vágvölgyi, C.; Pesti, M.

In: Folia Microbiologica, Vol. 48, No. 2, 03.2003, p. 203-209.

Research output: Contribution to journalArticle

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