Characterization of an Aspergillus nidulans L-arabitol dehydrogenase mutant

Ronald P. de Vries, M. Flipphi, Cor F B Witteveen, Jaap Visser

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The degradation pathway for L-arabinose, which consists of a sequence of alternating reduction and oxidation reactions prior to ultimate phosphorylation, was studied in Aspergillus nidulans wild-type as well as in an L-arabinose non-utilizing mutant. The inability of the mutant to use L-arabinose was caused by the absence of L-arabitol dehydrogenase activity. The effect of the mutation on polyol accumulation patterns was studied upon growth on various carbon sources. The presence of L-arabinose resulted in intracellular accumulation of arabitol in this mutant. Moreover, the mutant secreted arabitol under these conditions and, in contrast to the wild-type, featured enhanced expression of enzymes involved in L-arabinose catabolism as well as of extracellular glycosyl hydrolases involved in degradation of the plant cell wall polysaccharide L-arabinan.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalFEMS Microbiology Letters
Volume123
Issue number1-2
Publication statusPublished - Oct 15 1994

Fingerprint

Aspergillus nidulans
Arabinose
Oxidoreductases
Plant Cells
Hydrolases
Cell Wall
Polysaccharides
Carbon
Phosphorylation
arabitol
Mutation
Enzymes
Growth

Keywords

  • Arabinase expression
  • Aspergillus nidulans
  • Carbon catabolite repression
  • Induction
  • L-Arabinose atabolism
  • L-Arabitol dehydrogenase
  • Polyol accumulation

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

de Vries, R. P., Flipphi, M., Witteveen, C. F. B., & Visser, J. (1994). Characterization of an Aspergillus nidulans L-arabitol dehydrogenase mutant. FEMS Microbiology Letters, 123(1-2), 83-90.

Characterization of an Aspergillus nidulans L-arabitol dehydrogenase mutant. / de Vries, Ronald P.; Flipphi, M.; Witteveen, Cor F B; Visser, Jaap.

In: FEMS Microbiology Letters, Vol. 123, No. 1-2, 15.10.1994, p. 83-90.

Research output: Contribution to journalArticle

de Vries, RP, Flipphi, M, Witteveen, CFB & Visser, J 1994, 'Characterization of an Aspergillus nidulans L-arabitol dehydrogenase mutant', FEMS Microbiology Letters, vol. 123, no. 1-2, pp. 83-90.
de Vries, Ronald P. ; Flipphi, M. ; Witteveen, Cor F B ; Visser, Jaap. / Characterization of an Aspergillus nidulans L-arabitol dehydrogenase mutant. In: FEMS Microbiology Letters. 1994 ; Vol. 123, No. 1-2. pp. 83-90.
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