Characterization of an Aspergillus nidulans L-arabitol dehydrogenase mutant

Ronald P. de Vries, Michel J.A. Flipphi, Cor F.B. Witteveen, Jaap Visser

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The degradation pathway for L-arabinose, which consists of a sequence of alternating reduction and oxidation reactions prior to ultimate phosphorylation, was studied in Aspergillus nidulans wild-type as well as in an L-arabinose non-utilizing mutant. The inability of the mutant to use L-arabinose was caused by the absence of L-arabitol dehydrogenase activity. The effect of the mutation on polyol accumulation patterns was studied upon growth on various carbon sources. The presence of L-arabinose resulted in intracellular accumulation of arabitol in this mutant. Moreover, the mutant secreted arabitol under these conditions and, in contrast to the wild-type, featured enhanced expression of enzymes involved in L-arabinose catabolism as well as of extracellular glycosyl hydrolases involved in degradation of the plant cell wall polysaccharide L-arabinan.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalFEMS microbiology letters
Volume123
Issue number1-2
DOIs
Publication statusPublished - Oct 15 1994

Keywords

  • Arabinase expression
  • Aspergillus nidulans
  • Carbon catabolite repression
  • Induction
  • L-Arabinose atabolism
  • L-Arabitol dehydrogenase
  • Polyol accumulation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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