Characterization of a novel long-chain n-alkane-degrading strain, Dietzia sp. e1

Zoltán Bihari, Zsolt Szabó, Attila Szvetnik, Margit Balázsa, Péter Bartos, Péter Tolmacsov, Zoltán Zombori, I. Kiss

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The newly isolated strain E1, identifi ed as a Dietzia sp., proved to have an excellent ability to degrade n-C12 to n-C38 alkane components of crude oil. The preferred substrate was the very long-chain alkane n-eicosane at an optimal -temperature of 37 °C and an optimal pH of 8 under aerobic conditions. The growth and substrate uptake kinetics were monitored during the n-alkane fermentation process, and Dietzia sp. E1 cells were found to possess three distinct levels of cell-surface hydrophobicity. Gas chromatographic/mass spectrometric analysis revealed that intracellular substrate mineralization occurred through the conversion of n-alkane to the corresponding n-alkanal. The monoterminal oxidation pathway was presumably initiated by AlkB and CYP153 terminal alkane hydroxylases, both of their partial coding sequences were successfully detected in the genome of strain E1, a novel member of the Dietzia genus.

Original languageEnglish
Pages (from-to)693-700
Number of pages8
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume65 C
Issue number11-12
Publication statusPublished - 2010

Fingerprint

Alkanes
Substrates
Cytochrome P-450 CYP4A
Enzyme kinetics
Petroleum
Hydrophobicity
Hydrophobic and Hydrophilic Interactions
Fermentation
Genes
Gases
Genome
Oxidation
Temperature
Growth

Keywords

  • Cell-surface hydrophobicity
  • Dietzia
  • N-alkane

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Bihari, Z., Szabó, Z., Szvetnik, A., Balázsa, M., Bartos, P., Tolmacsov, P., ... Kiss, I. (2010). Characterization of a novel long-chain n-alkane-degrading strain, Dietzia sp. e1. Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 65 C(11-12), 693-700.

Characterization of a novel long-chain n-alkane-degrading strain, Dietzia sp. e1. / Bihari, Zoltán; Szabó, Zsolt; Szvetnik, Attila; Balázsa, Margit; Bartos, Péter; Tolmacsov, Péter; Zombori, Zoltán; Kiss, I.

In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences, Vol. 65 C, No. 11-12, 2010, p. 693-700.

Research output: Contribution to journalArticle

Bihari, Z, Szabó, Z, Szvetnik, A, Balázsa, M, Bartos, P, Tolmacsov, P, Zombori, Z & Kiss, I 2010, 'Characterization of a novel long-chain n-alkane-degrading strain, Dietzia sp. e1', Zeitschrift fur Naturforschung - Section C Journal of Biosciences, vol. 65 C, no. 11-12, pp. 693-700.
Bihari Z, Szabó Z, Szvetnik A, Balázsa M, Bartos P, Tolmacsov P et al. Characterization of a novel long-chain n-alkane-degrading strain, Dietzia sp. e1. Zeitschrift fur Naturforschung - Section C Journal of Biosciences. 2010;65 C(11-12):693-700.
Bihari, Zoltán ; Szabó, Zsolt ; Szvetnik, Attila ; Balázsa, Margit ; Bartos, Péter ; Tolmacsov, Péter ; Zombori, Zoltán ; Kiss, I. / Characterization of a novel long-chain n-alkane-degrading strain, Dietzia sp. e1. In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences. 2010 ; Vol. 65 C, No. 11-12. pp. 693-700.
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