Characterization of a new mitochondrial plasmid from Fusarium proliferatum

Miklós Láday, Veronika Stubnya, Z. Hamari, L. Hornok

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A 10.3 kb linear mitochondrial DNA plasmid designated pFP1 was isolated from Fusarium proliferatum. The DNA sequence of the plasmid consists of 10,336 bp with perfect terminal inverted repeats of 400 bp. Two major, non-overlapping ORFs were identified on opposite strands, encoding a phage-type RNA polymerase and a family B type DNA polymerase, respectively. One additional minor ORF encoding a putative highly basic protein was also identified. The copy number of pFP1, as determined by RT-PCR, ranged between 1.8 and 3.1 per mtDNA copies depending on the host strain. Real-time PCR analysis of a total of 400 cultures surviving ethidium bromide curing indicated that no plasmid-free strains could be obtained by this treatment. Further single spore selections of the survivors with reduced plasmid content were needed to obtain plasmid-free clones. No phenotypic differences were found between the wild-type strains and their plasmid-free progenies.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalPlasmid
Volume59
Issue number2
DOIs
Publication statusPublished - Mar 2008

Fingerprint

Fusarium
Plasmids
Mitochondrial DNA
Open Reading Frames
Ethidium
Terminal Repeat Sequences
DNA-Directed DNA Polymerase
DNA-Directed RNA Polymerases
Spores
Bacteriophages
Real-Time Polymerase Chain Reaction
Clone Cells
Polymerase Chain Reaction
Proteins

Keywords

  • Distribution
  • Ethidium bromide curing
  • Fusarium
  • Mitochondrial plasmid

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Characterization of a new mitochondrial plasmid from Fusarium proliferatum. / Láday, Miklós; Stubnya, Veronika; Hamari, Z.; Hornok, L.

In: Plasmid, Vol. 59, No. 2, 03.2008, p. 127-133.

Research output: Contribution to journalArticle

Láday, Miklós ; Stubnya, Veronika ; Hamari, Z. ; Hornok, L. / Characterization of a new mitochondrial plasmid from Fusarium proliferatum. In: Plasmid. 2008 ; Vol. 59, No. 2. pp. 127-133.
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