Characterization of a natural Plum pox virus isolate bearing a truncated coat protein

Erzsébet Szathmáry, Júlia Novák Nádudvari, László Szabó, István Tóbiás, E. Balázs, László Palkovics

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Plum pox virus (PPV) isolates were collected in Hungary from plum varieties. PCR targeting the 3′ genomic region resulted in a shorter PCR product in the case of the B1298 isolate bearing a 135-nucleotide deletion in frame in the N-terminal part of the coat protein (CP). The isolate was aphid-transmissible and the virion diameter was reduced compared to PPV-SK68. Detectability of this isolate by Western blot varied according to the antibody used. Integration of the deleted CP gene into an infectious PPV clone had no effect on infectivity and symptomatology. In competition experiments, B1298 had a considerable advantage in virus accumulation.

Original languageEnglish
Pages (from-to)141-145
Number of pages5
JournalArchives of Virology
Volume154
Issue number1
DOIs
Publication statusPublished - Jan 2009

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Plum Pox Virus
Capsid Proteins
Polymerase Chain Reaction
Aphids
Hungary
Virion
Nucleotides
Clone Cells
Western Blotting
Viruses
Antibodies
Genes

ASJC Scopus subject areas

  • Virology

Cite this

Szathmáry, E., Nádudvari, J. N., Szabó, L., Tóbiás, I., Balázs, E., & Palkovics, L. (2009). Characterization of a natural Plum pox virus isolate bearing a truncated coat protein. Archives of Virology, 154(1), 141-145. https://doi.org/10.1007/s00705-008-0281-9

Characterization of a natural Plum pox virus isolate bearing a truncated coat protein. / Szathmáry, Erzsébet; Nádudvari, Júlia Novák; Szabó, László; Tóbiás, István; Balázs, E.; Palkovics, László.

In: Archives of Virology, Vol. 154, No. 1, 01.2009, p. 141-145.

Research output: Contribution to journalArticle

Szathmáry, E, Nádudvari, JN, Szabó, L, Tóbiás, I, Balázs, E & Palkovics, L 2009, 'Characterization of a natural Plum pox virus isolate bearing a truncated coat protein', Archives of Virology, vol. 154, no. 1, pp. 141-145. https://doi.org/10.1007/s00705-008-0281-9
Szathmáry, Erzsébet ; Nádudvari, Júlia Novák ; Szabó, László ; Tóbiás, István ; Balázs, E. ; Palkovics, László. / Characterization of a natural Plum pox virus isolate bearing a truncated coat protein. In: Archives of Virology. 2009 ; Vol. 154, No. 1. pp. 141-145.
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