Characterization of a β-glucosidase with transgalactosylation capacity from the zygomycete Rhizomucor miehei

Judit Krisch, Ottó Bencsik, Tamás Papp, Csaba Vágvölgyi, Miklós Takó

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42 Citations (Scopus)

Abstract

An extracellular β-glucosidase from the zygomycete Rhizomucor miehei NRRL 5282 cultivated in a wheat bran-based solid state fermentation system was characterized. The purified enzyme exhibited an optimum temperature of 68-70°C and pH of 5.0. It efficiently hydrolyzed oligosaccharides having β-(1→4) glycosidic linkages and exhibited some β- and α-galactosidase activity. The V max for p-nitrophenyl-β-d-glucopyranoside and cellobiose was 468.2 and 115.5U/mg, respectively, while the K m was 0.12mM for both substrates. The enzyme had transglucosylation and transgalactosylation activities resulting in the formation of glycosides from cellobiose, lactose and ethanol. The enzyme increased the amounts of free phenolic antioxidants in sour cherry pomace indicating that its hydrolyzing activity could potentially be applicable to improve the bioavailability of these compounds.

Original languageEnglish
Pages (from-to)555-560
Number of pages6
JournalBioresource Technology
Volume114
DOIs
Publication statusPublished - Jun 1 2012

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Keywords

  • Extracellular β-glucosidase
  • Phenolic antioxidants
  • Rhizomucor miehei
  • Transgalactosylation
  • Transglucosylation

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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