Characterization and TLC - Bioautographic detection of essential oils from sone Thymus taxa. Determination of the activity of the oils and their components against plant pathogenic bacteria

Györgyi Horváth, László Gy Szabó, Éva Lemberkovics, Lajos Botz, Béla Kocsis

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The chemical composition of the oils of four thyme (Lamiaceae) chemotypes (Thymus vulgaris L., Thymus serpyllum L., Thymus × citriodorus (Pers.) Schreb., and Thymus × citriodorus " Archer's Gold") has been determined by gas chromatography (GC). Thymol was the main component of the oils of Thymus vulgaris and Thymus serpyllum, geraniol was the main component of the oil of Thymus × citriodorus, and carvacrol was the main component of the oil of Thymus × citriodorus "Archer's Gold". The bioactivity of the volatile oil of Thymus vulgaris and of the three main components of the oils against Gram negative plant pathogenic bacteria was examined by direct bioautography. They had an inhibitory effect on all of the test microorganisms. Two bacterial strains (Xanthomonas campestris pv. vesicatoria and Pseudomonas syringae pv. phaseolicola) were most sensitive in the bioautographic system and use of these bacteria would considerably shorten the process of bioautographic detection. The two antibiotics gentamycin and streptomycin were used as controls.

Original languageEnglish
Pages (from-to)300-304
Number of pages5
JournalJournal of Planar Chromatography - Modern TLC
Volume17
Issue number4
DOIs
Publication statusPublished - Jul 1 2004

Keywords

  • Direct bioautography
  • Essential oil
  • Gas chromatography, GC
  • Plant pathogenic bacteria
  • Thin layer chromatography, TLC
  • Thymus taxa

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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