Characterization and stimuli for production of pericardial, fluid atrial natriuretic peptide in dogs

I. Szokodi, F. Horkay, Pál Kiss, L. Selmeci, B. Merkely, V. Kékesi, Olli Vuolteenaho, Juhani Leppäluoto, Heikki Ruskoaho, A. Juhász-Nagy, Miklós Tóth

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Recently high immunoreactive atrial natriuretic peptide (ir-ANP) levels have been found in the pericardial fluid of patients undergoing cardiac surgery. The present study was designed to characterize pericardial fluid ANP in anesthetized dogs. Pericardial fluid ir-ANP levels were 3.4-fold higher than plasma levels and the molecular form, revealed by high performance liquid chromatography, was indistinguishable from ANP[99-126]. Elimination of [125I]ANP was 5-fold slower in the pericardial space than in plasma. Activity of the major ANP degrading enzyme, neutral endopeptidase NEP, EC 3.4.24.11), was 15-times higher in the pericardial fluid than in plasma. Right atrial balloon distension and rapid right ventricular pacing induced maximally 2.3-fold and 1.5-fold increases of pericardial fluid ir-ANP, respectively. Pericardial fluid ir-ANP concentrations and right atrial pressure values showed significant correlation during the stimuli. Our present results show that high concentrations of ir-ANP can be found in the dog pericardial fluid even under unstimulated conditions. Slow elimination of ANP from the pericardial fluid compartment may contribute to the high peptide levels. However this slow elimination cannot be attributed to a lower NEP activity. High basal levels of ANP in the pericardial fluid could be further increased by atrial balloon stretch and rapid ventricular pacing. The increase of pericardial fluid ir-ANP appeared to be a stretch-dependent response. ANP released into the pericardial fluid may be involved in the regulation of cardiac function and coronary vascular tone.

Original languageEnglish
Pages (from-to)1349-1359
Number of pages11
JournalLife Sciences
Volume61
Issue number14
DOIs
Publication statusPublished - Nov 29 1997

Fingerprint

Atrial Natriuretic Factor
Dogs
Fluids
Balloons
Plasmas
Pericardial Fluid
Neprilysin
Atrial Pressure
Pericardium
High performance liquid chromatography
Surgery
Thoracic Surgery
Blood Vessels
High Pressure Liquid Chromatography

Keywords

  • Atrial balloon distension
  • Atrial neuropeptide
  • Half-life
  • Neutral endopeptidase (EC 3.4.24.11)
  • Pericardial fluid
  • Ventricular pacing

ASJC Scopus subject areas

  • Pharmacology

Cite this

Characterization and stimuli for production of pericardial, fluid atrial natriuretic peptide in dogs. / Szokodi, I.; Horkay, F.; Kiss, Pál; Selmeci, L.; Merkely, B.; Kékesi, V.; Vuolteenaho, Olli; Leppäluoto, Juhani; Ruskoaho, Heikki; Juhász-Nagy, A.; Tóth, Miklós.

In: Life Sciences, Vol. 61, No. 14, 29.11.1997, p. 1349-1359.

Research output: Contribution to journalArticle

Szokodi, I. ; Horkay, F. ; Kiss, Pál ; Selmeci, L. ; Merkely, B. ; Kékesi, V. ; Vuolteenaho, Olli ; Leppäluoto, Juhani ; Ruskoaho, Heikki ; Juhász-Nagy, A. ; Tóth, Miklós. / Characterization and stimuli for production of pericardial, fluid atrial natriuretic peptide in dogs. In: Life Sciences. 1997 ; Vol. 61, No. 14. pp. 1349-1359.
@article{d20509ad32de4ef5b3a6f613ffb4164d,
title = "Characterization and stimuli for production of pericardial, fluid atrial natriuretic peptide in dogs",
abstract = "Recently high immunoreactive atrial natriuretic peptide (ir-ANP) levels have been found in the pericardial fluid of patients undergoing cardiac surgery. The present study was designed to characterize pericardial fluid ANP in anesthetized dogs. Pericardial fluid ir-ANP levels were 3.4-fold higher than plasma levels and the molecular form, revealed by high performance liquid chromatography, was indistinguishable from ANP[99-126]. Elimination of [125I]ANP was 5-fold slower in the pericardial space than in plasma. Activity of the major ANP degrading enzyme, neutral endopeptidase NEP, EC 3.4.24.11), was 15-times higher in the pericardial fluid than in plasma. Right atrial balloon distension and rapid right ventricular pacing induced maximally 2.3-fold and 1.5-fold increases of pericardial fluid ir-ANP, respectively. Pericardial fluid ir-ANP concentrations and right atrial pressure values showed significant correlation during the stimuli. Our present results show that high concentrations of ir-ANP can be found in the dog pericardial fluid even under unstimulated conditions. Slow elimination of ANP from the pericardial fluid compartment may contribute to the high peptide levels. However this slow elimination cannot be attributed to a lower NEP activity. High basal levels of ANP in the pericardial fluid could be further increased by atrial balloon stretch and rapid ventricular pacing. The increase of pericardial fluid ir-ANP appeared to be a stretch-dependent response. ANP released into the pericardial fluid may be involved in the regulation of cardiac function and coronary vascular tone.",
keywords = "Atrial balloon distension, Atrial neuropeptide, Half-life, Neutral endopeptidase (EC 3.4.24.11), Pericardial fluid, Ventricular pacing",
author = "I. Szokodi and F. Horkay and P{\'a}l Kiss and L. Selmeci and B. Merkely and V. K{\'e}kesi and Olli Vuolteenaho and Juhani Lepp{\"a}luoto and Heikki Ruskoaho and A. Juh{\'a}sz-Nagy and Mikl{\'o}s T{\'o}th",
year = "1997",
month = "11",
day = "29",
doi = "10.1016/S0024-3205(97)00680-2",
language = "English",
volume = "61",
pages = "1349--1359",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "14",

}

TY - JOUR

T1 - Characterization and stimuli for production of pericardial, fluid atrial natriuretic peptide in dogs

AU - Szokodi, I.

AU - Horkay, F.

AU - Kiss, Pál

AU - Selmeci, L.

AU - Merkely, B.

AU - Kékesi, V.

AU - Vuolteenaho, Olli

AU - Leppäluoto, Juhani

AU - Ruskoaho, Heikki

AU - Juhász-Nagy, A.

AU - Tóth, Miklós

PY - 1997/11/29

Y1 - 1997/11/29

N2 - Recently high immunoreactive atrial natriuretic peptide (ir-ANP) levels have been found in the pericardial fluid of patients undergoing cardiac surgery. The present study was designed to characterize pericardial fluid ANP in anesthetized dogs. Pericardial fluid ir-ANP levels were 3.4-fold higher than plasma levels and the molecular form, revealed by high performance liquid chromatography, was indistinguishable from ANP[99-126]. Elimination of [125I]ANP was 5-fold slower in the pericardial space than in plasma. Activity of the major ANP degrading enzyme, neutral endopeptidase NEP, EC 3.4.24.11), was 15-times higher in the pericardial fluid than in plasma. Right atrial balloon distension and rapid right ventricular pacing induced maximally 2.3-fold and 1.5-fold increases of pericardial fluid ir-ANP, respectively. Pericardial fluid ir-ANP concentrations and right atrial pressure values showed significant correlation during the stimuli. Our present results show that high concentrations of ir-ANP can be found in the dog pericardial fluid even under unstimulated conditions. Slow elimination of ANP from the pericardial fluid compartment may contribute to the high peptide levels. However this slow elimination cannot be attributed to a lower NEP activity. High basal levels of ANP in the pericardial fluid could be further increased by atrial balloon stretch and rapid ventricular pacing. The increase of pericardial fluid ir-ANP appeared to be a stretch-dependent response. ANP released into the pericardial fluid may be involved in the regulation of cardiac function and coronary vascular tone.

AB - Recently high immunoreactive atrial natriuretic peptide (ir-ANP) levels have been found in the pericardial fluid of patients undergoing cardiac surgery. The present study was designed to characterize pericardial fluid ANP in anesthetized dogs. Pericardial fluid ir-ANP levels were 3.4-fold higher than plasma levels and the molecular form, revealed by high performance liquid chromatography, was indistinguishable from ANP[99-126]. Elimination of [125I]ANP was 5-fold slower in the pericardial space than in plasma. Activity of the major ANP degrading enzyme, neutral endopeptidase NEP, EC 3.4.24.11), was 15-times higher in the pericardial fluid than in plasma. Right atrial balloon distension and rapid right ventricular pacing induced maximally 2.3-fold and 1.5-fold increases of pericardial fluid ir-ANP, respectively. Pericardial fluid ir-ANP concentrations and right atrial pressure values showed significant correlation during the stimuli. Our present results show that high concentrations of ir-ANP can be found in the dog pericardial fluid even under unstimulated conditions. Slow elimination of ANP from the pericardial fluid compartment may contribute to the high peptide levels. However this slow elimination cannot be attributed to a lower NEP activity. High basal levels of ANP in the pericardial fluid could be further increased by atrial balloon stretch and rapid ventricular pacing. The increase of pericardial fluid ir-ANP appeared to be a stretch-dependent response. ANP released into the pericardial fluid may be involved in the regulation of cardiac function and coronary vascular tone.

KW - Atrial balloon distension

KW - Atrial neuropeptide

KW - Half-life

KW - Neutral endopeptidase (EC 3.4.24.11)

KW - Pericardial fluid

KW - Ventricular pacing

UR - http://www.scopus.com/inward/record.url?scp=0030771808&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030771808&partnerID=8YFLogxK

U2 - 10.1016/S0024-3205(97)00680-2

DO - 10.1016/S0024-3205(97)00680-2

M3 - Article

C2 - 9335224

AN - SCOPUS:0030771808

VL - 61

SP - 1349

EP - 1359

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 14

ER -