Characterization and heterologous expression of an age-dependent fungal/bacterial type chitinase of Aspergillus nidulans

Éva Erdei, Tünde Pusztahelyi, M. Miskei, Teréz Barna, I. Pócsi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Under carbon starvation, Aspergillus nidulans produced a fungal/bacterial type chitinase, ChiB. The chiB gene was cloned and subcloned into pJC40 expression vector containing a 10XHis fusion tag, and the ChiB protein was expressed heterologously in Escherichia coli. Recombinant and native ChiB enzymes shared the same optimal pH ranges and showed similar substrate specificities with endo-acting cleavage patterns.

Original languageEnglish
Pages (from-to)351-361
Number of pages11
JournalActa microbiologica et immunologica Hungarica
Volume55
Issue number3
DOIs
Publication statusPublished - Sep 1 2008

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Keywords

  • Aspergillus nidulans
  • Autolysis
  • Endochitinase
  • Heterologous expression

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

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