Characterization and function of histamine receptors in human bone marrow stromal cells

Krisztian Nemeth, Todd Wilson, Balazs Rada, Alissa Parmelee, Balazs Mayer, Edit Buzas, Andras Falus, Sharon Key, Tamas Masszi, Sarolta Karpati, Eva Mezey

Research output: Contribution to journalArticle

19 Citations (Scopus)


There are several clinical trials worldwide using bone marrow stromal cells (BMSCs) as a cellular therapy to modulate immune responses in patients suffering from various inflammatory conditions. A deeper understanding of the molecular mechanisms involved in this modulatory effect could help us design better, more effective protocols to treat immune mediated diseases. In this study, we demonstrated that human BMSCs express H1, H2, and H4 histamine receptors and they respond to histamine stimulation with an increased interleukin 6 (IL-6) production both in vitro and in vivo. Using different receptor antagonists, we pinpointed the importance of the H1 histamine receptor, while Western blot analysis and application of various mitogen-activated protein kinase inhibitors highlighted the role of p38, extracellular signal-regulated kinase, and c-Jun N-terminal kinase kinases in the observed effect. When BMSCs were pretreated with either histamine or degranulated human mast cells, they exhibited an enhanced IL-6-dependent antiapoptotic effect on neutrophil granulocytes. Based on these observations, it is likely that introduction of BMSCs into a histamine-rich environment (such as any allergic setting) or pretreatment of these cells with synthetic histamine could have a significant modulatory effect on the therapeutic potential of BMSCs.

Original languageEnglish
Pages (from-to)222-231
Number of pages10
JournalStem Cells
Issue number2
Publication statusPublished - Feb 1 2012


  • Adult stem cells
  • Apoptosis
  • Bone marrow stromal cells
  • IL-6R/IL-6
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Characterization and function of histamine receptors in human bone marrow stromal cells'. Together they form a unique fingerprint.

  • Cite this