Characterization and chromosome location of the mouse link protein gene (Crtl1)

F. Deák, L. Mátés, K. Krysan, Z. Liu, P. E. Szabó, J. R. Mann, D. R. Beier, I. Kiss

Research output: Contribution to journalArticle

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Abstract

Link protein (LP) plays an essential role in endochondral bone formation by stabilizing the supramolecular assemblies of aggrecan and hyaluronan. We have isolated and characterized the mouse link protein gene (Crtl1). It is longer than 40 kb and transcribed from two alternative promoters, leading to heterogenous mRNAs between 5.3 and 1.3 kb in size. Apart from the coding sequence, the 5' flanking region is also highly conserved in mammals. Immunostaining revealed high levels of LP expression in the cartilaginous primordia of skeletal elements and low levels in other tissues. Using single-strand conformation polymorphism analysis, Crtl1 was assigned to mouse chromosome 13, tightly linked to Dhfr.

Original languageEnglish
Pages (from-to)75-79
Number of pages5
JournalCytogenetics and Cell Genetics
Volume87
Issue number1-2
Publication statusPublished - 1999

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Chromosomes
Genes
Aggrecans
Chromosomes, Human, Pair 13
5' Flanking Region
Hyaluronic Acid
Osteogenesis
Mammals
Messenger RNA
link protein

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Characterization and chromosome location of the mouse link protein gene (Crtl1). / Deák, F.; Mátés, L.; Krysan, K.; Liu, Z.; Szabó, P. E.; Mann, J. R.; Beier, D. R.; Kiss, I.

In: Cytogenetics and Cell Genetics, Vol. 87, No. 1-2, 1999, p. 75-79.

Research output: Contribution to journalArticle

Deák, F, Mátés, L, Krysan, K, Liu, Z, Szabó, PE, Mann, JR, Beier, DR & Kiss, I 1999, 'Characterization and chromosome location of the mouse link protein gene (Crtl1)', Cytogenetics and Cell Genetics, vol. 87, no. 1-2, pp. 75-79.
Deák, F. ; Mátés, L. ; Krysan, K. ; Liu, Z. ; Szabó, P. E. ; Mann, J. R. ; Beier, D. R. ; Kiss, I. / Characterization and chromosome location of the mouse link protein gene (Crtl1). In: Cytogenetics and Cell Genetics. 1999 ; Vol. 87, No. 1-2. pp. 75-79.
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