Characteristics of the ventricular transient outward potassium current in genetic rodent models of diabetes

Z. Rusznák, Z. Cseresnyés, I. Sipos, J. Magyar, L. Kovács, G. Szücs

Research output: Contribution to journalArticle

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Abstract

The whole-cell configuration of the patch-clamp technique was employed to measure the transient outward potassium current in enzymatically isolated ventricular cells of spontaneously diabetic rats (BB/Wor) and mice (ob/ob). Healthy littermates (non-diabetic BB rats and lean mice) were used as controls. There was no significant difference between the non-diabetic and diabetic BB rats (Type I diabetes, IDDM) in the amplitude of either the current measured in the absence or the one found in the presence of 4-aminopyridine. The voltage dependence of the activation and steady-state inactivation was also similar in both populations, as no significant difference was observed in the rate of recovery from inactivation of Ito. The amplitudes of the total and 4-aminopyridine sensitive currents of lean and obese mice (Type II diabetes, NIDDM) were also similar. The voltage dependences of the activation and of the steady-state inactivation did not differ significantly, either. Our results might indicate certain limitations of the applicability of experiments carried out on genetically diabetic rats if the results are compared to those derived from the healthy littermates as controls.

Original languageEnglish
Pages (from-to)225-238
Number of pages14
JournalGeneral Physiology and Biophysics
Volume15
Issue number3
Publication statusPublished - Jun 1996

Fingerprint

Inbred BB Rats
Genetic Models
Rodentia
4-Aminopyridine
Potassium
Type 1 Diabetes Mellitus
Type 2 Diabetes Mellitus
Obese Mice
Patch-Clamp Techniques
Population

Keywords

  • 4-aminopyridine
  • BB/Wor rat
  • Ob/ob mouse
  • Transient outward potassium current
  • Ventricular cardiomyocytes

ASJC Scopus subject areas

  • Biophysics
  • Physiology

Cite this

Characteristics of the ventricular transient outward potassium current in genetic rodent models of diabetes. / Rusznák, Z.; Cseresnyés, Z.; Sipos, I.; Magyar, J.; Kovács, L.; Szücs, G.

In: General Physiology and Biophysics, Vol. 15, No. 3, 06.1996, p. 225-238.

Research output: Contribution to journalArticle

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