Characteristics of endocrine pancreas in chronic pancreatitis, as revealed by simultaneous immunocytochemical demonstration of hormone production

T. Krenács, E. Dobo, Z. Laszik

Research output: Contribution to journalArticle

Abstract

Cells producing insulin, glucagon, somatostatin, and pancreatic polypeptide (PP) were demonstrated by simultaneous immunocytochemical detection in human pancreatic sections of 3 patients with chronic pancreatitis (CP) and diabetes mellitus (DM), 2 patients with CP and normal glucose tolerance, and 2 patients with normal morphology (3 sections each). The triple and quadruple labeling protocol involved the sequential use of immunogold silver staining (IGSS), indirect immunoalkaline phosphatase (IAP), the indirect immunoperoxidase (IPO), and the peroxidase-antiperoxidase (PAP) methods. This approach proved useful for the recognition of some topo-morphological features of the endocrine pancreas at a glance in normal and CP tissues. In the process of islet cell neoformation (ductoendocrine proliferation), the A cells seemed to occur first within the ducts and ductules, followed by the B and D cells. Additionally, in some islets of Langerhans damaged by intraislet peliosis in DM patients, the A cells were found to be very resistant, whereas the number B cells was seriously reduced.

Original languageEnglish
Pages (from-to)213-218
Number of pages6
JournalJournal of Histotechnology
Volume13
Issue number3
Publication statusPublished - 1990

Fingerprint

Chronic Pancreatitis
Islets of Langerhans
Hormones
Diabetes Mellitus
B-Lymphocytes
Pancreatic Polypeptide
Silver Staining
Purpura
Somatostatin-Secreting Cells
Somatostatin
Glucagon
Phosphoric Monoester Hydrolases
Peroxidase
Cell Proliferation
Insulin
Glucose

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

@article{693b54d6916049c89c9d1a41112d2ed4,
title = "Characteristics of endocrine pancreas in chronic pancreatitis, as revealed by simultaneous immunocytochemical demonstration of hormone production",
abstract = "Cells producing insulin, glucagon, somatostatin, and pancreatic polypeptide (PP) were demonstrated by simultaneous immunocytochemical detection in human pancreatic sections of 3 patients with chronic pancreatitis (CP) and diabetes mellitus (DM), 2 patients with CP and normal glucose tolerance, and 2 patients with normal morphology (3 sections each). The triple and quadruple labeling protocol involved the sequential use of immunogold silver staining (IGSS), indirect immunoalkaline phosphatase (IAP), the indirect immunoperoxidase (IPO), and the peroxidase-antiperoxidase (PAP) methods. This approach proved useful for the recognition of some topo-morphological features of the endocrine pancreas at a glance in normal and CP tissues. In the process of islet cell neoformation (ductoendocrine proliferation), the A cells seemed to occur first within the ducts and ductules, followed by the B and D cells. Additionally, in some islets of Langerhans damaged by intraislet peliosis in DM patients, the A cells were found to be very resistant, whereas the number B cells was seriously reduced.",
author = "T. Kren{\'a}cs and E. Dobo and Z. Laszik",
year = "1990",
language = "English",
volume = "13",
pages = "213--218",
journal = "Journal of Histotechnology",
issn = "0147-8885",
publisher = "Maney Publishing",
number = "3",

}

TY - JOUR

T1 - Characteristics of endocrine pancreas in chronic pancreatitis, as revealed by simultaneous immunocytochemical demonstration of hormone production

AU - Krenács, T.

AU - Dobo, E.

AU - Laszik, Z.

PY - 1990

Y1 - 1990

N2 - Cells producing insulin, glucagon, somatostatin, and pancreatic polypeptide (PP) were demonstrated by simultaneous immunocytochemical detection in human pancreatic sections of 3 patients with chronic pancreatitis (CP) and diabetes mellitus (DM), 2 patients with CP and normal glucose tolerance, and 2 patients with normal morphology (3 sections each). The triple and quadruple labeling protocol involved the sequential use of immunogold silver staining (IGSS), indirect immunoalkaline phosphatase (IAP), the indirect immunoperoxidase (IPO), and the peroxidase-antiperoxidase (PAP) methods. This approach proved useful for the recognition of some topo-morphological features of the endocrine pancreas at a glance in normal and CP tissues. In the process of islet cell neoformation (ductoendocrine proliferation), the A cells seemed to occur first within the ducts and ductules, followed by the B and D cells. Additionally, in some islets of Langerhans damaged by intraislet peliosis in DM patients, the A cells were found to be very resistant, whereas the number B cells was seriously reduced.

AB - Cells producing insulin, glucagon, somatostatin, and pancreatic polypeptide (PP) were demonstrated by simultaneous immunocytochemical detection in human pancreatic sections of 3 patients with chronic pancreatitis (CP) and diabetes mellitus (DM), 2 patients with CP and normal glucose tolerance, and 2 patients with normal morphology (3 sections each). The triple and quadruple labeling protocol involved the sequential use of immunogold silver staining (IGSS), indirect immunoalkaline phosphatase (IAP), the indirect immunoperoxidase (IPO), and the peroxidase-antiperoxidase (PAP) methods. This approach proved useful for the recognition of some topo-morphological features of the endocrine pancreas at a glance in normal and CP tissues. In the process of islet cell neoformation (ductoendocrine proliferation), the A cells seemed to occur first within the ducts and ductules, followed by the B and D cells. Additionally, in some islets of Langerhans damaged by intraislet peliosis in DM patients, the A cells were found to be very resistant, whereas the number B cells was seriously reduced.

UR - http://www.scopus.com/inward/record.url?scp=0025027228&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025027228&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0025027228

VL - 13

SP - 213

EP - 218

JO - Journal of Histotechnology

JF - Journal of Histotechnology

SN - 0147-8885

IS - 3

ER -