Characteristics of Cu deficiency-induced inhibition of photosynthetic electron transport in spinach chloroplasts

Magdolna Droppa, Jiři Masojidek, Zsuzsanna Rózsa, Adam Wolak, LászlóI Horváth, T. Farkas, G. Horváth

Research output: Contribution to journalArticle

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Abstract

In the present work we studied the effect of Cu deficiency on spinach chloroplasts. We found that in spinach the electron transport was inhibited as reported previously for sugar beet (Droppa, M., Terry, N. and Horváth, G. (1984) Proc. Natl. Acad. Sci. USA (1984) 81, 2369-2373). The breakpoint of the Arrhenius plot of the whole electron-transport activity was shifted from +6°C to +12°C in Cu-deficient chloroplasts. A similar effect could be observed with a spin-labelled probe, when the rotational correlation time was plotted vs. the reciprocal temperatures. This indicates that the membrane fluidity might be changed by Cu deficiency. The lipid/protein ratios were similar in both control and deficient chloroplasts. On the other hand, the saturated/unsaturated ratio of phosphatidylcholine (PC), phosphatidylglycerol (PG) and sulpholipids (SL) was increased but that of monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) decreased. We conclude that Cu deficiency does not change the entire membrane fluidity but rather the lipid composition of the microenvironment of some electron-transport components. The inhibition of Photosystem II electron transport in Cu-deficient chloroplasts was characterized by thermoluminescence and 2-dimensional gel electrophoresis. It was found that Cu deficiency shifted the main peak of the glow curve from +18°C to +8°C, similar to that of DCMU-poisoned chloroplasts. Two apoproteins of the 29 kDa polypeptide disappeared in Cu-deficient chloroplasts which indicates that this polypeptide has a regulatory role in ensuring the normal electron flow between QA and QB.

Original languageEnglish
Pages (from-to)75-84
Number of pages10
JournalBBA - Bioenergetics
Volume891
Issue number1
DOIs
Publication statusPublished - Mar 25 1987

Fingerprint

Spinacia oleracea
Chloroplasts
Electron Transport
Fluidity
Membrane Fluidity
Diuron
Sugar beets
Membranes
Lipids
Arrhenius plots
Phosphatidylglycerols
Peptides
Apoproteins
Thermoluminescence
Photosystem II Protein Complex
Electrophoresis
Phosphatidylcholines
Beta vulgaris
Gels
Electrons

Keywords

  • Chloroplast membrane
  • Copper deficiency
  • Membrane fluidity
  • Photosynthetic electron transport
  • Photosystem II polypeptide

ASJC Scopus subject areas

  • Biophysics

Cite this

Characteristics of Cu deficiency-induced inhibition of photosynthetic electron transport in spinach chloroplasts. / Droppa, Magdolna; Masojidek, Jiři; Rózsa, Zsuzsanna; Wolak, Adam; Horváth, LászlóI; Farkas, T.; Horváth, G.

In: BBA - Bioenergetics, Vol. 891, No. 1, 25.03.1987, p. 75-84.

Research output: Contribution to journalArticle

Droppa, Magdolna ; Masojidek, Jiři ; Rózsa, Zsuzsanna ; Wolak, Adam ; Horváth, LászlóI ; Farkas, T. ; Horváth, G. / Characteristics of Cu deficiency-induced inhibition of photosynthetic electron transport in spinach chloroplasts. In: BBA - Bioenergetics. 1987 ; Vol. 891, No. 1. pp. 75-84.
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AU - Masojidek, Jiři

AU - Rózsa, Zsuzsanna

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AB - In the present work we studied the effect of Cu deficiency on spinach chloroplasts. We found that in spinach the electron transport was inhibited as reported previously for sugar beet (Droppa, M., Terry, N. and Horváth, G. (1984) Proc. Natl. Acad. Sci. USA (1984) 81, 2369-2373). The breakpoint of the Arrhenius plot of the whole electron-transport activity was shifted from +6°C to +12°C in Cu-deficient chloroplasts. A similar effect could be observed with a spin-labelled probe, when the rotational correlation time was plotted vs. the reciprocal temperatures. This indicates that the membrane fluidity might be changed by Cu deficiency. The lipid/protein ratios were similar in both control and deficient chloroplasts. On the other hand, the saturated/unsaturated ratio of phosphatidylcholine (PC), phosphatidylglycerol (PG) and sulpholipids (SL) was increased but that of monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) decreased. We conclude that Cu deficiency does not change the entire membrane fluidity but rather the lipid composition of the microenvironment of some electron-transport components. The inhibition of Photosystem II electron transport in Cu-deficient chloroplasts was characterized by thermoluminescence and 2-dimensional gel electrophoresis. It was found that Cu deficiency shifted the main peak of the glow curve from +18°C to +8°C, similar to that of DCMU-poisoned chloroplasts. Two apoproteins of the 29 kDa polypeptide disappeared in Cu-deficient chloroplasts which indicates that this polypeptide has a regulatory role in ensuring the normal electron flow between QA and QB.

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