1. 1. T and B lymphocytes from human tonsils were separated on a nylon wool column. T cells are enriched in the nonadherent, B cells in the adherent fraction. 2. 2. Several enzymes and other markers were tested in separated and non separated lymphocyte populations. Certain enzymes and other properties can be used as T or B lymphocyte markers because of their preferential occurrence and because of the advantages of their estimation (simple, quick methods, objective evaluation). 3. 3. The following characteristics were considered as markers on the basis of our results: (I) acid phosphatase, Na+K+-activated ATPase, BAEE-peptidase and chromium labeling in T lymphocytes; (II) 5′-nucleotidase, FITC-IgG binding, N-acetyl-d-glucosaminidase, thymidine and valine incorporation in B lymphocytes.
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