Characterisation of the swine swC1 antigen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Some biochemical and functional characteristics of the swine swC1 antigen, determined by the use of the authors' swC1-specific monoclonal antibody (mAb) 335-2, are reported. The molecular weight of the antigen was determined by immunoprecipitation. The swC1 antigen has 41 and approx. 15 kD components under reducing conditions. It is sensitive to proteolytic enzymes such as bromelain or trypsin, but not to papain. Phosphatidylinositol-specific phospholipase C treatment diminished the expression of swCl on the surface of leukocytes. Cross-linking of swC1 on the cell surface did not influence the proliferation of mitogen-activated mononuclear cells and had no mitogenic activity by itself. During 48 h of mitogen activation its surface expression did not change significantly. Possible relationships of swC1 to human CD antigens are discussed in the light of the results obtained.

Original languageEnglish
Pages (from-to)17-31
Number of pages15
JournalActa Veterinaria Hungarica
Volume45
Issue number1
Publication statusPublished - 1997

Fingerprint

Swine
antigens
Mitogens
Antigens
swine
CD antigens
CD Antigens
bromelains
Bromelains
Phosphoinositide Phospholipase C
Papain
phospholipase C
papain
phosphatidylinositols
crosslinking
Immunoprecipitation
Trypsin
trypsin
functional properties
leukocytes

Keywords

  • Cell surface antigens
  • Immunoprecipitation
  • PI-anchor
  • swC1
  • Swine leukocytes

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Characterisation of the swine swC1 antigen. / Magyar, A.; Mihalik, R.

In: Acta Veterinaria Hungarica, Vol. 45, No. 1, 1997, p. 17-31.

Research output: Contribution to journalArticle

@article{41f0c2566e1b456facb3e2f0dcc9691f,
title = "Characterisation of the swine swC1 antigen",
abstract = "Some biochemical and functional characteristics of the swine swC1 antigen, determined by the use of the authors' swC1-specific monoclonal antibody (mAb) 335-2, are reported. The molecular weight of the antigen was determined by immunoprecipitation. The swC1 antigen has 41 and approx. 15 kD components under reducing conditions. It is sensitive to proteolytic enzymes such as bromelain or trypsin, but not to papain. Phosphatidylinositol-specific phospholipase C treatment diminished the expression of swCl on the surface of leukocytes. Cross-linking of swC1 on the cell surface did not influence the proliferation of mitogen-activated mononuclear cells and had no mitogenic activity by itself. During 48 h of mitogen activation its surface expression did not change significantly. Possible relationships of swC1 to human CD antigens are discussed in the light of the results obtained.",
keywords = "Cell surface antigens, Immunoprecipitation, PI-anchor, swC1, Swine leukocytes",
author = "A. Magyar and R. Mihalik",
year = "1997",
language = "English",
volume = "45",
pages = "17--31",
journal = "Acta Veterinaria Hungarica",
issn = "0236-6290",
publisher = "Akademiai Kiado",
number = "1",

}

TY - JOUR

T1 - Characterisation of the swine swC1 antigen

AU - Magyar, A.

AU - Mihalik, R.

PY - 1997

Y1 - 1997

N2 - Some biochemical and functional characteristics of the swine swC1 antigen, determined by the use of the authors' swC1-specific monoclonal antibody (mAb) 335-2, are reported. The molecular weight of the antigen was determined by immunoprecipitation. The swC1 antigen has 41 and approx. 15 kD components under reducing conditions. It is sensitive to proteolytic enzymes such as bromelain or trypsin, but not to papain. Phosphatidylinositol-specific phospholipase C treatment diminished the expression of swCl on the surface of leukocytes. Cross-linking of swC1 on the cell surface did not influence the proliferation of mitogen-activated mononuclear cells and had no mitogenic activity by itself. During 48 h of mitogen activation its surface expression did not change significantly. Possible relationships of swC1 to human CD antigens are discussed in the light of the results obtained.

AB - Some biochemical and functional characteristics of the swine swC1 antigen, determined by the use of the authors' swC1-specific monoclonal antibody (mAb) 335-2, are reported. The molecular weight of the antigen was determined by immunoprecipitation. The swC1 antigen has 41 and approx. 15 kD components under reducing conditions. It is sensitive to proteolytic enzymes such as bromelain or trypsin, but not to papain. Phosphatidylinositol-specific phospholipase C treatment diminished the expression of swCl on the surface of leukocytes. Cross-linking of swC1 on the cell surface did not influence the proliferation of mitogen-activated mononuclear cells and had no mitogenic activity by itself. During 48 h of mitogen activation its surface expression did not change significantly. Possible relationships of swC1 to human CD antigens are discussed in the light of the results obtained.

KW - Cell surface antigens

KW - Immunoprecipitation

KW - PI-anchor

KW - swC1

KW - Swine leukocytes

UR - http://www.scopus.com/inward/record.url?scp=0031284841&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031284841&partnerID=8YFLogxK

M3 - Article

VL - 45

SP - 17

EP - 31

JO - Acta Veterinaria Hungarica

JF - Acta Veterinaria Hungarica

SN - 0236-6290

IS - 1

ER -