Characterisation of the nucleic acid binding features of the PRRSV 7ap and its ability to induce antinuclear antibodies

Ferenc Olasz, Béla Denes, Adám Balint, Tibor Magyar, Sándor Belak, Zoltán Zádori

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A short alternative open reading frame named ORF7a has recently been discovered within the nucleocapsid gene of the porcine reproductive and respiratory syndrome virus (PRRSV) genome. Proteins (7ap) translated from the ORF7a of two divergent strains -a type I and a type II -are able to completely reduce the motility of nucleic acids at relatively high molar charge ratios in gel retardation assays indicating strong dsDNA-and ssRNA-binding capability. Conserved RNAand DNA-binding properties suggest that nucleic acid binding is a functional property of the divergent 7aps, and not an arbitrary consequence of their net positive charge. Sera from Hu7ap-immunised pigs and mice did not react with Hu7ap or Hu7ap-GFP; however, antinuclear antibodies were detected in the sera of the immunised animals, suggesting an ability of Hu7ap to interact with or mimic autoantigenic macromolecules.

Original languageEnglish
Pages (from-to)124-134
Number of pages11
JournalActa veterinaria Hungarica
Volume65
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Keywords

  • 7ap proteins
  • Antinuclear antibodies
  • Nucleic acid binding features
  • ORF7a
  • Porcine reproductive and respiratory syndrome virus

ASJC Scopus subject areas

  • veterinary(all)

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