Characterisation of the large-scale production process of oyster mushroom (Pleurotus ostreatus) with the analysis of succession and spatial heterogeneity of lignocellulolytic enzyme activities

Renáta Bánfi, Zsuzsanna Pohner, József Kovács, Szabina Luzics, Adrienn Nagy, Melinda Dudás, Péter Tanos, K. Márialigeti, Balázs Vajna

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Oyster mushroom (Pleurotus ostreatus) lignocellulolytic enzyme activity pattern and variation was investigated in a large-scale facility from spawning until the end of the second flush. In the first cultivation cycle laccase production reached its peak during vegetative growth stage, while manganese-peroxidase showed the highest activity during fruiting body induction. Cellulose and hemicellulose degrading enzymes had maximal activity at the beginning of flush and harvest stage. The enzyme activities showed similar tendencies among five different mushroom substrate blocks representing a production house. The spatial variability analysis of enzyme activities pointed out the within substrate block heterogeneity as the main source if variation. This result was confirmed by Combined Cluster and Discriminant Analysis (CCDA) method showing minimal among block heterogeneity considering the whole investigation period; furthermore in the first cultivation cycle all blocks were grouped into one cluster.

Original languageEnglish
Pages (from-to)1354-1363
Number of pages10
JournalFungal Biology
Volume119
Issue number12
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

Pleurotus
Pleurotus ostreatus
Spatial Analysis
mushroom
mushrooms
oysters
enzyme activity
manganese peroxidase
Enzymes
substrate
laccase
fruiting
activity pattern
fruiting bodies
discriminant analysis
hemicellulose
spatial analysis
Laccase
vegetative growth
cluster analysis

Keywords

  • Analysis of variability
  • CCDA
  • Large-scale mushroom production
  • Lignocellulolytic enzyme activity
  • Pleurotus ostreatus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Genetics
  • Infectious Diseases

Cite this

Characterisation of the large-scale production process of oyster mushroom (Pleurotus ostreatus) with the analysis of succession and spatial heterogeneity of lignocellulolytic enzyme activities. / Bánfi, Renáta; Pohner, Zsuzsanna; Kovács, József; Luzics, Szabina; Nagy, Adrienn; Dudás, Melinda; Tanos, Péter; Márialigeti, K.; Vajna, Balázs.

In: Fungal Biology, Vol. 119, No. 12, 01.12.2015, p. 1354-1363.

Research output: Contribution to journalArticle

Bánfi, Renáta ; Pohner, Zsuzsanna ; Kovács, József ; Luzics, Szabina ; Nagy, Adrienn ; Dudás, Melinda ; Tanos, Péter ; Márialigeti, K. ; Vajna, Balázs. / Characterisation of the large-scale production process of oyster mushroom (Pleurotus ostreatus) with the analysis of succession and spatial heterogeneity of lignocellulolytic enzyme activities. In: Fungal Biology. 2015 ; Vol. 119, No. 12. pp. 1354-1363.
@article{fc323576207343ef9f1a56b44e02b0c4,
title = "Characterisation of the large-scale production process of oyster mushroom (Pleurotus ostreatus) with the analysis of succession and spatial heterogeneity of lignocellulolytic enzyme activities",
abstract = "Oyster mushroom (Pleurotus ostreatus) lignocellulolytic enzyme activity pattern and variation was investigated in a large-scale facility from spawning until the end of the second flush. In the first cultivation cycle laccase production reached its peak during vegetative growth stage, while manganese-peroxidase showed the highest activity during fruiting body induction. Cellulose and hemicellulose degrading enzymes had maximal activity at the beginning of flush and harvest stage. The enzyme activities showed similar tendencies among five different mushroom substrate blocks representing a production house. The spatial variability analysis of enzyme activities pointed out the within substrate block heterogeneity as the main source if variation. This result was confirmed by Combined Cluster and Discriminant Analysis (CCDA) method showing minimal among block heterogeneity considering the whole investigation period; furthermore in the first cultivation cycle all blocks were grouped into one cluster.",
keywords = "Analysis of variability, CCDA, Large-scale mushroom production, Lignocellulolytic enzyme activity, Pleurotus ostreatus",
author = "Ren{\'a}ta B{\'a}nfi and Zsuzsanna Pohner and J{\'o}zsef Kov{\'a}cs and Szabina Luzics and Adrienn Nagy and Melinda Dud{\'a}s and P{\'e}ter Tanos and K. M{\'a}rialigeti and Bal{\'a}zs Vajna",
year = "2015",
month = "12",
day = "1",
doi = "10.1016/j.funbio.2015.10.003",
language = "English",
volume = "119",
pages = "1354--1363",
journal = "Fungal Biology",
issn = "1878-6146",
publisher = "Elsevier",
number = "12",

}

TY - JOUR

T1 - Characterisation of the large-scale production process of oyster mushroom (Pleurotus ostreatus) with the analysis of succession and spatial heterogeneity of lignocellulolytic enzyme activities

AU - Bánfi, Renáta

AU - Pohner, Zsuzsanna

AU - Kovács, József

AU - Luzics, Szabina

AU - Nagy, Adrienn

AU - Dudás, Melinda

AU - Tanos, Péter

AU - Márialigeti, K.

AU - Vajna, Balázs

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Oyster mushroom (Pleurotus ostreatus) lignocellulolytic enzyme activity pattern and variation was investigated in a large-scale facility from spawning until the end of the second flush. In the first cultivation cycle laccase production reached its peak during vegetative growth stage, while manganese-peroxidase showed the highest activity during fruiting body induction. Cellulose and hemicellulose degrading enzymes had maximal activity at the beginning of flush and harvest stage. The enzyme activities showed similar tendencies among five different mushroom substrate blocks representing a production house. The spatial variability analysis of enzyme activities pointed out the within substrate block heterogeneity as the main source if variation. This result was confirmed by Combined Cluster and Discriminant Analysis (CCDA) method showing minimal among block heterogeneity considering the whole investigation period; furthermore in the first cultivation cycle all blocks were grouped into one cluster.

AB - Oyster mushroom (Pleurotus ostreatus) lignocellulolytic enzyme activity pattern and variation was investigated in a large-scale facility from spawning until the end of the second flush. In the first cultivation cycle laccase production reached its peak during vegetative growth stage, while manganese-peroxidase showed the highest activity during fruiting body induction. Cellulose and hemicellulose degrading enzymes had maximal activity at the beginning of flush and harvest stage. The enzyme activities showed similar tendencies among five different mushroom substrate blocks representing a production house. The spatial variability analysis of enzyme activities pointed out the within substrate block heterogeneity as the main source if variation. This result was confirmed by Combined Cluster and Discriminant Analysis (CCDA) method showing minimal among block heterogeneity considering the whole investigation period; furthermore in the first cultivation cycle all blocks were grouped into one cluster.

KW - Analysis of variability

KW - CCDA

KW - Large-scale mushroom production

KW - Lignocellulolytic enzyme activity

KW - Pleurotus ostreatus

UR - http://www.scopus.com/inward/record.url?scp=84947975925&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84947975925&partnerID=8YFLogxK

U2 - 10.1016/j.funbio.2015.10.003

DO - 10.1016/j.funbio.2015.10.003

M3 - Article

VL - 119

SP - 1354

EP - 1363

JO - Fungal Biology

JF - Fungal Biology

SN - 1878-6146

IS - 12

ER -