Characterisation of the first VIM metallo - Lactamase-producing Pseudomonas aeruginosa clinical isolate in Serbia

Zorica Lepsanovic, B. Libisch, Branka Tomanovic, Zorka Nonkovic, Boglrka Balogh, M. Füzi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

From the Central-East European region the first VIM metallo - lactamase (MBL) producing Pseudomonas aeruginosa strains were published from Croatia, Poland and Hungary. The aim of this study was to assess the contribution of MBL-production to carbapenem-resistance among P. aeruginosa clinical isolates in the Military Medical Academy (MMA) in Belgrade, Serbia between August 2004 and September 2007. Only one P. aeruginosa isolate with strain number 722 proved MBL-positive that harboured a novel class 1 integron with a bla VIM-2-like cassette in the first position, followed by orfD, a putative gene with unknown function. Our data indicate that MBL-producing strains occur at a prevalence of less than 1% among imipenemnonsusceptible P. aeruginosa clinical isolates in this Belgrade hospital. The newly identified VIM MBL-producing P. aeruginosa strain 722 could be assigned to serotype O11, and it was panresistant to all antimicrobials tested. The isolate displayed sequence type ST235 by multilocus sequence typing which is the founder sequence type of the previously identified international clonal complex CC11 that already contains bla VIM-positive isolates from Italy, Greece, Sweden, Hungary and Poland. In conclusion, this is the first report of VIM MBL-producing P. aeruginosa from Serbia and also of the occurrence of such isolates belonging to the international clonal complex CC11 in this country.

Original languageEnglish
Pages (from-to)447-454
Number of pages8
JournalActa Microbiologica et Immunologica Hungarica
Volume55
Issue number4
DOIs
Publication statusPublished - Dec 2008

Fingerprint

Serbia
Pseudomonas aeruginosa
Hungary
Poland
Integrons
Multilocus Sequence Typing
Croatia
Carbapenems
Greece
Sweden
Italy
Genes

Keywords

  • Carbapenem
  • CC11
  • Clonal complex
  • Integron
  • Metallo - lactamase
  • MLST
  • Pseudomonas aeruginosa
  • Serotype

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Characterisation of the first VIM metallo - Lactamase-producing Pseudomonas aeruginosa clinical isolate in Serbia. / Lepsanovic, Zorica; Libisch, B.; Tomanovic, Branka; Nonkovic, Zorka; Balogh, Boglrka; Füzi, M.

In: Acta Microbiologica et Immunologica Hungarica, Vol. 55, No. 4, 12.2008, p. 447-454.

Research output: Contribution to journalArticle

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