Characterisation of the first complete genome sequence of the roe deer (Capreolus capreolus) papillomavirus

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The complete genomic DNA of a novel roe deer (Capreolus capreolus) papillomavirus (CcPV1) was amplified and sequenced from fibropapillomatous skin lesions of a Hungarian roe deer. Viral DNA was detected by a pair of degenerate primers and the remaining genomic sequence was amplified by a long-template high-fidelity PCR and sequenced. The CcPV1 genome was 8032 bp long and contained open reading frames (ORFs) typical for Delta-papillomaviruses (E6, E7, E1, E2, E4, E5, E9, L2, and L1) and a 799 bp long untranslated regulatory region (URR). Phylogenetic analysis based on the 3861 bp long concatenated sequence of the E1-E2-L2-L1 ORFs and on separate alignments of all major ORFs using both neighbour-joining and maximum parsimony methods placed CcPV1 on a distinct branch between Ovine papillomavirus 1 and the other deer papillomaviruses within the Delta-papillomavirus genus, although pairwise nucleotide alignments of L1 ORF sequences determined highest identities with European Elk Papillomavirus (71.2%) and Reindeer Papillomavirus (70.3%).

Original languageEnglish
Pages (from-to)307-311
Number of pages5
JournalVirus Research
Volume135
Issue number2
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Deer
Open Reading Frames
Genome
Reindeer
Untranslated Regions
Nucleic Acid Regulatory Sequences
Viral DNA
Sheep
Nucleotides
Polymerase Chain Reaction
Skin
DNA

Keywords

  • Fibropapilloma
  • Genome
  • Papillomavirus
  • Roe deer
  • Skin
  • Tumour

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Characterisation of the first complete genome sequence of the roe deer (Capreolus capreolus) papillomavirus. / Erdélyi, K.; Bálint, A.; Dencso, László; Dán, A.; Ursu, K.

In: Virus Research, Vol. 135, No. 2, 08.2008, p. 307-311.

Research output: Contribution to journalArticle

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