Characterisation of specific activities and hydrolytic properties of cell-wall-degrading enzymes produced by Trichoderma reesei rut C30 on different carbon sources

Bálint Sipos, Zsuzsa Benkő, Dóra Dienes, Kati Réczey, Liisa Viikari, Matti Siika-Aho

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Conversion of lignocellulosic substrates is limited by several factors, in terms of both the enzymes and the substrates. Better understanding of the hydrolysis mechanisms and the factors determining their performance is crucial for commercial lignocellulosesbased processes. Enzymes produced on various carbon sources (Solka Floc 200, lactose and steam-pre-treated corn stover) by Trichoderma reesei Rut C30 were characterised by their enzyme profile and hydrolytic performance. The results showed that there was a clear correlation between the secreted amount of xylanase and mannanase enzymes and that their production was induced by the presence of xylan in the carbon source. Co-secretion of α-arabinosidase and α-galactosidase was also observed. Secretion of β-glucosidase was found to be clearly dependent on the composition of the carbon source, and in the case of lactose, 2-fold higher specific activity was observed compared to Solka Floc and steampre- treated corn stover. Hydrolysis experiments showed a clear connection between glucan and xylan conversion and highlighted the importance of β-glucosidase and xylanase activities. When hydrolysis was performed using additional purified β-glucosidase and xylanase, the addition of β-glucosidase was found to significantly improve both the xylan and glucan conversion.

Original languageEnglish
Pages (from-to)347-364
Number of pages18
JournalApplied Biochemistry and Biotechnology
Volume161
Issue number1-8
DOIs
Publication statusPublished - May 2010

Keywords

  • Accessory enzymes
  • Cellulase fermentation
  • Enzymatic hydrolysis
  • Hemicellulases
  • Trichoderma reesei Rut C30
  • β-Glucosidase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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