Characterisation of reversed-phase liquid chromatographic columns by chromatographic tests. Evaluation of 36 test parameters: Repeatability, reproducibility and correlation

Dóra Visky, Yvan Vander Heyden, Tímea Iványi, Peggy Baten, Jacques De Beer, Zsuzsanna Kovács, Béla Noszál, Eugène Roets, Désiré L. Massart, Jos Hoogmartens

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The European Pharmacopoeia (Ph. Eur.) or other official compendia give only a general description of the stationary phase in the description of a liquid chromatographic method. Therefore the selection of a column giving suitable selectivity presents difficulties. Earlier, a test procedure was proposed that allows measurement of a number of parameters which are reported to be representative for stationary phase characteristics. This paper describes how the test procedure was applied on 69 RP-LC C18 columns. Chromatographic parameters obtained as test results were evaluated, and their repeatability, reproducibility and correlation were examined.

Original languageEnglish
Pages (from-to)39-58
Number of pages20
JournalJournal of Chromatography A
Volume977
Issue number1
DOIs
Publication statusPublished - Nov 15 2002

Keywords

  • Chromatographic test
  • Column characterization
  • Stationary phases, LC
  • Test parameters

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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