Characterisation of genetic variation for aluminium resistance and polyphenol oxidase activity in genebank accessions of spelt wheat

Harsh Raman, Rummana Rahman, David Luckett, Rosy Raman, Ferenc Bekes, L. Láng, Z. Bedő

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Spelt wheat [Triticum aestivum ssp. spelta (L.) Thell.] is becoming a valuable crop due to its reputation as a healthy food. In Australia, this crop has not been targeted for systematic breeding. Identification of spelt genotypes having low PPO activity and resistance to acid soils (Al3+) are desirable attributes for future cultivar development. We evaluated 51 genebank accessions of spelts from the Australian Winter Cereals Collection for polyphenol oxidase (PPO) activity and for resistance to aluminium (Al). PPO activity was measured both visually and spectrophotometrically, using L-DOPA substrate. PPO activity for genotypes ranged from 0.15 to 1.3 and could be grouped into 'low', 'medium' and 'high' categories. At least eight acces- sions exhibited low PPO activity (not different to the durum check cultivar Arrivato). After measuring PPO activity, the same kernels were further evaluated for Al resistance using a nutrient solution culture method with haematoxylin staining test of root tips. Thirty-three accessions were resistant to Al. Functional gene markers associated with loci conditioning Al resistance gene (TaALMT1) and PPO activity (XPPO-2A) in common wheat confirmed their association with target phenotypes within spelt accessions. Genetic variation within spelt wheat for important agronomic and quality traits such as Al resistance and PPO suggested that progress in spelt improvement can be made by molecular plant breeding. Diversity Array Technology (DArT) based allelic data revealed that these spelt accessions are genetically diverse.

Original languageEnglish
Pages (from-to)373-381
Number of pages9
JournalBreeding Science
Volume59
Issue number4
DOIs
Publication statusPublished - 2009

Fingerprint

Triticum aestivum subsp. spelta
Catechol Oxidase
Aluminum
catechol oxidase
Triticum
aluminum
genetic variation
Genotype
DNA Shuffling
Meristem
genotype
cultivars
Hematoxylin
crops
plant breeding
durum wheat
root tips
acid soils
Genes
nutrient solutions

Keywords

  • Aluminium resistance
  • Genetic resources
  • Molecular diversity
  • Polyphenol oxidase
  • Triticum aestivum ssp. spelta

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Characterisation of genetic variation for aluminium resistance and polyphenol oxidase activity in genebank accessions of spelt wheat. / Raman, Harsh; Rahman, Rummana; Luckett, David; Raman, Rosy; Bekes, Ferenc; Láng, L.; Bedő, Z.

In: Breeding Science, Vol. 59, No. 4, 2009, p. 373-381.

Research output: Contribution to journalArticle

Raman, Harsh ; Rahman, Rummana ; Luckett, David ; Raman, Rosy ; Bekes, Ferenc ; Láng, L. ; Bedő, Z. / Characterisation of genetic variation for aluminium resistance and polyphenol oxidase activity in genebank accessions of spelt wheat. In: Breeding Science. 2009 ; Vol. 59, No. 4. pp. 373-381.
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