Characterisation of eGFP-transgenic BALB/c mouse strain established by lentiviral transgenesis

K. Kvell, T. Czömpöly, László Hiripi, P. Balogh, József Kóbor, Lilla Bodrogi, J. Pongrácz, William A. Ritchie, Z. Bösze

Research output: Contribution to journalArticle

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Abstract

Lentiviral technology is a powerful tool for the creation of stable transgenic animals. However, uncertainties have remained whether constitutive promoters resist long-term silencing. We used concentrated HIV-1 based lentiviral vectors to create stable transgenic BALB/c mice by perivitelline injection. In our vectors eGFP expression was driven by the human EF1α promoter. The established transgenic animals were analyzed for eGFP expression by in vivo fluorescence imaging, PCR, histology and flow-cytometry. eGFP expression showed even distribution without mosaicism; however, tissue-dependent differences of eGFP expression were observed. Up to the sixth generation only one newborn showed eGFP inactivation. eGFP + transgenic bone marrow cells efficiently provided long-term haemopoietic repopulation in radiation chimeras, regenerating all bone marrow-derived lineages with eGFP + cells with distinct eGFP expression profiles. The established eGFP + BALB/c mouse strain is expected to be extremely useful in various immunological experiments.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalTransgenic Research
Volume19
Issue number1
DOIs
Publication statusPublished - Jan 2010

Fingerprint

Gene Transfer Techniques
transgenic animals
Genetically Modified Animals
Radiation Chimera
promoter regions
genetically modified organisms
Mosaicism
chimerism
Optical Imaging
bone marrow cells
mice
Human immunodeficiency virus 1
Bone Marrow Cells
bone marrow
histology
Uncertainty
flow cytometry
HIV-1
Histology
Flow Cytometry

Keywords

  • Balb/c
  • Bone-marrow chimeric mouse
  • eGFP
  • Lentiviral transgenesis

ASJC Scopus subject areas

  • Biotechnology
  • Genetics
  • Agronomy and Crop Science
  • Animal Science and Zoology

Cite this

Characterisation of eGFP-transgenic BALB/c mouse strain established by lentiviral transgenesis. / Kvell, K.; Czömpöly, T.; Hiripi, László; Balogh, P.; Kóbor, József; Bodrogi, Lilla; Pongrácz, J.; Ritchie, William A.; Bösze, Z.

In: Transgenic Research, Vol. 19, No. 1, 01.2010, p. 105-112.

Research output: Contribution to journalArticle

Kvell, K. ; Czömpöly, T. ; Hiripi, László ; Balogh, P. ; Kóbor, József ; Bodrogi, Lilla ; Pongrácz, J. ; Ritchie, William A. ; Bösze, Z. / Characterisation of eGFP-transgenic BALB/c mouse strain established by lentiviral transgenesis. In: Transgenic Research. 2010 ; Vol. 19, No. 1. pp. 105-112.
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