Characterisation of community structur of bacteria in parallel mesophilic and thermophilic pilot scale anaerobe sludge digesters

T. Tauber, Brigitta Berta, Anna J. Székely, I. Gyarmati, Katalin Kékesi, K. Márialigeti, Erika M. Tóth

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The aim of the present work was to compare the microbial communities of a mesophilic and a thermophilic pilot scale anaerobe sludge digester. For studying the communities cultivation independent chemotaxonomical methods (RQ and PLFA analyses) and T-RFLP were applied. Microbial communities of the mesophilic and thermophilic pilot digesters showed considerable differences, both concerning the species present, and their abundance. A Methanosarcina sp. dominated the thermophilic, while a Methanosaeta sp. the mesophilic digester among Archaea. Species diversity of Bacteria was reduced in the thermophilic digester. Based on the quinone patterns in both digesters the dominance of sulphate reducing respiratory bacteria could be detected. The PLFA profiles of the digester communities were similar though in minor components characteristic differences were shown. Level of branched chain fatty acids is slightly lower in the thermophilic digester that reports less Gram positive bacteria. The relative ratio of fatty acids characteristic to Enterobacteriaceae, Bacteroidetes and Clostridia shows differences between the two digesters: their importance generally decreased under thermophilic conditions. The sulphate reducer marker (15:1 and 17:1) fatty acids are present in low quantity in both digesters.

Original languageEnglish
Pages (from-to)47-55
Number of pages9
JournalActa microbiologica et immunologica Hungarica
Volume54
Issue number1
DOIs
Publication statusPublished - Mar 1 2007

Keywords

  • Chemotaxonomic analysis
  • Community structure
  • Sludge digester
  • T-RFLP

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

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