Characterisation of community structur of bacteria in parallel mesophilic and thermophilic pilot scale anaerobe sludge digesters

T. Tauber, Brigitta Berta, Anna J. Székely, I. Gyarmati, K. Kékesi, K. Márialigeti, E. Tóth

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The aim of the present work was to compare the microbial communities of a mesophilic and a thermophilic pilot scale anaerobe sludge digester. For studying the communities cultivation independent chemotaxonomical methods (RQ and PLFA analyses) and T-RFLP were applied. Microbial communities of the mesophilic and thermophilic pilot digesters showed considerable differences, both concerning the species present, and their abundance. A Methanosarcina sp. dominated the thermophilic, while a Methanosaeta sp. the mesophilic digester among Archaea. Species diversity of Bacteria was reduced in the thermophilic digester. Based on the quinone patterns in both digesters the dominance of sulphate reducing respiratory bacteria could be detected. The PLFA profiles of the digester communities were similar though in minor components characteristic differences were shown. Level of branched chain fatty acids is slightly lower in the thermophilic digester that reports less Gram positive bacteria. The relative ratio of fatty acids characteristic to Enterobacteriaceae, Bacteroidetes and Clostridia shows differences between the two digesters: their importance generally decreased under thermophilic conditions. The sulphate reducer marker (15:1 and 17:1) fatty acids are present in low quantity in both digesters.

Original languageEnglish
Pages (from-to)47-55
Number of pages9
JournalActa Microbiologica et Immunologica Hungarica
Volume54
Issue number1
DOIs
Publication statusPublished - Mar 2007

Fingerprint

Sewage
Fatty Acids
Bacteria
Sulfates
Methanosarcina
Bacteroidetes
Clostridium
Archaea
Gram-Positive Bacteria
Enterobacteriaceae
Restriction Fragment Length Polymorphisms

Keywords

  • Chemotaxonomic analysis
  • Community structure
  • Sludge digester
  • T-RFLP

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Medicine(all)
  • Microbiology

Cite this

Characterisation of community structur of bacteria in parallel mesophilic and thermophilic pilot scale anaerobe sludge digesters. / Tauber, T.; Berta, Brigitta; Székely, Anna J.; Gyarmati, I.; Kékesi, K.; Márialigeti, K.; Tóth, E.

In: Acta Microbiologica et Immunologica Hungarica, Vol. 54, No. 1, 03.2007, p. 47-55.

Research output: Contribution to journalArticle

@article{b596523824fd43c392c2faf7c3678454,
title = "Characterisation of community structur of bacteria in parallel mesophilic and thermophilic pilot scale anaerobe sludge digesters",
abstract = "The aim of the present work was to compare the microbial communities of a mesophilic and a thermophilic pilot scale anaerobe sludge digester. For studying the communities cultivation independent chemotaxonomical methods (RQ and PLFA analyses) and T-RFLP were applied. Microbial communities of the mesophilic and thermophilic pilot digesters showed considerable differences, both concerning the species present, and their abundance. A Methanosarcina sp. dominated the thermophilic, while a Methanosaeta sp. the mesophilic digester among Archaea. Species diversity of Bacteria was reduced in the thermophilic digester. Based on the quinone patterns in both digesters the dominance of sulphate reducing respiratory bacteria could be detected. The PLFA profiles of the digester communities were similar though in minor components characteristic differences were shown. Level of branched chain fatty acids is slightly lower in the thermophilic digester that reports less Gram positive bacteria. The relative ratio of fatty acids characteristic to Enterobacteriaceae, Bacteroidetes and Clostridia shows differences between the two digesters: their importance generally decreased under thermophilic conditions. The sulphate reducer marker (15:1 and 17:1) fatty acids are present in low quantity in both digesters.",
keywords = "Chemotaxonomic analysis, Community structure, Sludge digester, T-RFLP",
author = "T. Tauber and Brigitta Berta and Sz{\'e}kely, {Anna J.} and I. Gyarmati and K. K{\'e}kesi and K. M{\'a}rialigeti and E. T{\'o}th",
year = "2007",
month = "3",
doi = "10.1556/AMicr.54.2007.1.5",
language = "English",
volume = "54",
pages = "47--55",
journal = "Acta Microbiologica et Immunologica Hungarica",
issn = "1217-8950",
publisher = "Akademiai Kiado",
number = "1",

}

TY - JOUR

T1 - Characterisation of community structur of bacteria in parallel mesophilic and thermophilic pilot scale anaerobe sludge digesters

AU - Tauber, T.

AU - Berta, Brigitta

AU - Székely, Anna J.

AU - Gyarmati, I.

AU - Kékesi, K.

AU - Márialigeti, K.

AU - Tóth, E.

PY - 2007/3

Y1 - 2007/3

N2 - The aim of the present work was to compare the microbial communities of a mesophilic and a thermophilic pilot scale anaerobe sludge digester. For studying the communities cultivation independent chemotaxonomical methods (RQ and PLFA analyses) and T-RFLP were applied. Microbial communities of the mesophilic and thermophilic pilot digesters showed considerable differences, both concerning the species present, and their abundance. A Methanosarcina sp. dominated the thermophilic, while a Methanosaeta sp. the mesophilic digester among Archaea. Species diversity of Bacteria was reduced in the thermophilic digester. Based on the quinone patterns in both digesters the dominance of sulphate reducing respiratory bacteria could be detected. The PLFA profiles of the digester communities were similar though in minor components characteristic differences were shown. Level of branched chain fatty acids is slightly lower in the thermophilic digester that reports less Gram positive bacteria. The relative ratio of fatty acids characteristic to Enterobacteriaceae, Bacteroidetes and Clostridia shows differences between the two digesters: their importance generally decreased under thermophilic conditions. The sulphate reducer marker (15:1 and 17:1) fatty acids are present in low quantity in both digesters.

AB - The aim of the present work was to compare the microbial communities of a mesophilic and a thermophilic pilot scale anaerobe sludge digester. For studying the communities cultivation independent chemotaxonomical methods (RQ and PLFA analyses) and T-RFLP were applied. Microbial communities of the mesophilic and thermophilic pilot digesters showed considerable differences, both concerning the species present, and their abundance. A Methanosarcina sp. dominated the thermophilic, while a Methanosaeta sp. the mesophilic digester among Archaea. Species diversity of Bacteria was reduced in the thermophilic digester. Based on the quinone patterns in both digesters the dominance of sulphate reducing respiratory bacteria could be detected. The PLFA profiles of the digester communities were similar though in minor components characteristic differences were shown. Level of branched chain fatty acids is slightly lower in the thermophilic digester that reports less Gram positive bacteria. The relative ratio of fatty acids characteristic to Enterobacteriaceae, Bacteroidetes and Clostridia shows differences between the two digesters: their importance generally decreased under thermophilic conditions. The sulphate reducer marker (15:1 and 17:1) fatty acids are present in low quantity in both digesters.

KW - Chemotaxonomic analysis

KW - Community structure

KW - Sludge digester

KW - T-RFLP

UR - http://www.scopus.com/inward/record.url?scp=34250669165&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250669165&partnerID=8YFLogxK

U2 - 10.1556/AMicr.54.2007.1.5

DO - 10.1556/AMicr.54.2007.1.5

M3 - Article

C2 - 17523391

AN - SCOPUS:34250669165

VL - 54

SP - 47

EP - 55

JO - Acta Microbiologica et Immunologica Hungarica

JF - Acta Microbiologica et Immunologica Hungarica

SN - 1217-8950

IS - 1

ER -