Characterisation of avian Pasteurella multocida strains with PCR-RFLP analysis of the ompH gene

Boglárka Sellyei, Éva Ivanics, Tibor Magyar

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The 16 somatic serotype type strains and 60 field isolates of Pasteurella multocida, representing various avian species and geographic regions in Hungary, were characterised by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the ompH gene with DraI restriction endonuclease. The type strains yielded eight different (I-VIII) profiles. Strains whose PCR fragment was uncut by DraI (profile IV) could be differentiated with HindIII and PvuII restriction endonucleases. Five of the eight PCR-RFLP profiles (I, III, V, VI and VII) were detected among the field strains. Only a correlation of limited strength was found between the classical somatic serotypes and the PCR-RFLP profiles. However, the results confirmed that molecular methods could confidently distinguish serotype A:1 strains from the other serotypes. Moreover, the specific relationship between somatic serotypes and PCR-RFLP types among isolates from turkey raises the possibility of the existence of host-specific clones within the P. multocida population.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalActa veterinaria Hungarica
Volume61
Issue number1
DOIs
Publication statusPublished - Mar 1 2013

    Fingerprint

Keywords

  • PCR-RFLP
  • Pasteurella multocida
  • Somatic serotype
  • ompH

ASJC Scopus subject areas

  • veterinary(all)

Cite this