Characterisation and comparison of avian pasteurella multocida strains by conventional and eric-pcr assays

Boglárka Sellyei, Zsuzsanna Varga, E. Ivánics, Tibor Magyar

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Sixty-one avian strains of Pasteurella multocida were characterised and compared by biochemical tests, capsular PCR typing and ERIC-PCR. The strains were recovered from various avian species (goose, duck, Muscovy duck, turkey, chicken and pheasant) and represented different geographic locations in Hungary. Forty-two strains (69%) were identified as P. multocida subsp. multocida and 19 strains (31%) as P. multocida subsp. septica. The strains were grouped into 7 different biovars (1, 2, 3, 4, 5, 6 and 7). The most prevalent biovars were 1 (25%), 3 (21%) and 6 (21%). Most of the duck isolates (90%) belonged to biovar 1 or 6. The most frequent capsular type was A (93.5%). Type F represented only a small number (6.5%) of the strains. Other capsular types were not identified. From the 61 isolates 24 different fingerprint patterns were generated by ERIC-PCR assay. Based on cluster analysis the strains could be grouped into four larger and four miniclusters that showed considerable correlation with the geographical origin and the host species. The results indicate that ERIC-PCR may be a suitable technique for studying the host adaptation of P. multocida and the epidemiology of fowl cholera.

Original languageEnglish
Pages (from-to)429-440
Number of pages12
JournalActa Veterinaria Hungarica
Volume56
Issue number4
DOIs
Publication statusPublished - Dec 2008

Fingerprint

Pasteurella multocida
Ducks
Polymerase Chain Reaction
assays
Geese
Geographic Locations
Hungary
Cholera
Dermatoglyphics
ducks
Pasteurella multocida subsp. septica
Pasteurella multocida subsp. multocida
Cluster Analysis
Chickens
Epidemiology
fowl cholera
Cairina moschata
pheasants
geese
epidemiology

Keywords

  • Biovar
  • Capsular type
  • ERIC-PCR
  • Pasteurella multocida
  • Poultry

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Characterisation and comparison of avian pasteurella multocida strains by conventional and eric-pcr assays. / Sellyei, Boglárka; Varga, Zsuzsanna; Ivánics, E.; Magyar, Tibor.

In: Acta Veterinaria Hungarica, Vol. 56, No. 4, 12.2008, p. 429-440.

Research output: Contribution to journalArticle

Sellyei, Boglárka ; Varga, Zsuzsanna ; Ivánics, E. ; Magyar, Tibor. / Characterisation and comparison of avian pasteurella multocida strains by conventional and eric-pcr assays. In: Acta Veterinaria Hungarica. 2008 ; Vol. 56, No. 4. pp. 429-440.
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