Changes of the cAMP-dependent protein kinases during differentiation of lens cells

Attila TakÁts, Ferenc Antoni, Anna FaragÓ

Research output: Contribution to journalArticle

Abstract

Several changes occur in the properties of cAMP-dependent protein kinase during the differentiation of lens epithelial cells, and/or in the course of aging of fiber cells. Two types of isoenzymes (I and II) were found on DEAE-cellulose chromatography of epithelial cells. The chromatography of the crude extract of cortical fibers yielded one protein kinase fraction only, in the position of type I kinase. A sharp cAMP-dependent protein kinase peak was observed in the position of isoenzyme II on DEAE, and several lower molecular weight, cAMP-dependent protein kinase fractions appeared in Sephadex G-200 after mild acid treatment (pH 4.8) of the cortical crude extract.3H-cAMP saturation curve (Scatchard plot) of acid-treaied protein kinase showed the loss of positive cooperativity. Aggregation of the I and II isoenzymes during lens cell differentiation is suggested.

Original languageEnglish
Pages (from-to)366-371
Number of pages6
JournalOphthalmic Research
Volume11
Issue number5-6
DOIs
Publication statusPublished - Jan 1 1979

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Keywords

  • Aggregation of enzyme molecules
  • CAMP-dependent protein kinase
  • Fractionation of protein kinase
  • Isoenzymes
  • Lens cell differentiation

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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