Changes of spontaneous and induced platelet aggregation in the presence of a human endothelial cell monolayer

I. Altorjay, C. M. Kirchmaier, H. K. Breddin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A new, simple method has been developed for the investigation of platelet aggregation in the presence of a cultured confluent human endothelial cell monolayer, in disk shaped rotating cuvettes, without magnetic stirring. The endothelial cells inhibited the aggregating effect of several inducers in a concentration dependent manner. At a platelet count of 5×105/μl in PRP e.g. the aggregating effect of 1 μg/ml collagen, 10-6 M adrenalin, 10-6 M ADP and 0.2 U/ml thrombin was completely abolished. Spontaneous aggregation was also prevented by the EC-monolayer. A correlation could be established between the inhibitory effect of ECs and the number of platelets. In PRP with a platelet count of 7×10-5/μl the inhibitory effect of the endothelial cell monolayer significantly decreased.

Original languageEnglish
Pages (from-to)69-77
Number of pages9
JournalThrombosis Research
Volume55
Issue number1
DOIs
Publication statusPublished - Jul 1 1989

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Platelet Count
Endothelial Cells
Platelet Aggregation
Thrombin
Adenosine Diphosphate
Epinephrine
Collagen
Spontaneous Platelet Aggregation

Keywords

  • ADP
  • adrenalin
  • collagen
  • Endothelial cell culture
  • platelet aggregation
  • thrombin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Changes of spontaneous and induced platelet aggregation in the presence of a human endothelial cell monolayer. / Altorjay, I.; Kirchmaier, C. M.; Breddin, H. K.

In: Thrombosis Research, Vol. 55, No. 1, 01.07.1989, p. 69-77.

Research output: Contribution to journalArticle

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