Changes of prostacyclin and thromboxane synthesis in the course of mouse liver perfusion. Stimulated thromboxane A2 synthesis of freshly prepared isolated mouse hepatocytes

Z. Spolarics, I. Mucha, G. Bánhegyi, T. Garzó, R. Machovich, F. Antoni, J. Mandl

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The formation of prostacyclin and thromboxane A2 (measured as 6-keto PGF and TXB2 by radioimmunoassay) was investigated during a 30 min perfusion of mouse liver in a recirculation system. After cannulation of the portal vein an immediate increase of de novo synthesis and secretion of PGI2 occured followed by a sharp decrease. Increased PGI2 synthesis was also followed by a continuous increase of TXA2 synthesis and secretion reaching a maximum at the end of the 30 min perfusion. Elevated TXA2 synthesis was also shown in freshly isolated hepatocytes investigated in the course of a 20 min incubation period immediately after the perfusion. However, the elevated TXA2 formation was not observed when it was measured after a 120 min preincubation of the cells. Both PGI2 and TXA2 production could be provoked to a similar extent by the addition of arachidonate and A 23187 immediately after the perfusion or after a 120 min preincubation.

Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Volume36
Issue number2
DOIs
Publication statusPublished - May 1989

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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