Changes of local and systemic hemorheological properties in intestinal ischemia-reperfusion injury in the rat model

E. Bráth, N. Németh, Ferenc Kiss, Erika Sajtos, Timea Hever, Lili Matyas, L. Tóth, I. Mikó, I. Furka

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Abstract

The aim of this study was to investigate intestinal ischemia-reperfusion and its local and systemic hemorheological relations in the rat. Ten anaesthetized female CD outbred rats were equally divided into 2 experimental groups. (1) Ischemia-reperfusion (I/R): the superior mesenterial artery was clipped for 30 minutes. After removing the clip, 60 minutes of the reperfusion was observed before extermination. Blood samples were taken from the caudal caval vein and from the portal vein before ischemia, 1 minute before and after clip removal, and at the 15th, 30th, and 60th minutes of the reperfusion. (2) Sham operation: median laparotomy and blood sampling were done according to the timing as in I/R group. Hematological parameters, red blood cell aggregation, and deformability were determined. Leukocyte count and mean volume of erythrocytes increased slightly but continuously in portal venous samples during the reperfusion period. Red blood cell aggregation values were higher in portal blood by the end of ischemia, and then became elevated further comparing to the caval venous blood. Both in caval and portal venous samples of I/R group red blood cell deformability significantly worsened during the experimental period compared to its base and Sham group. In portal blood red blood cell deformability was impaired more than in caval vein samples. Histology showed denuded villi, dilated capillaries, and the inflammatory cells were increased after a 30 minutes ischemia. In conclusion, intestinal ischemia-reperfusion causes changes in erythrocyte deformability and aggregation, showing local versus systemic differences in venous blood during the first hour of reperfusion.

Original languageEnglish
Pages (from-to)321-326
Number of pages6
JournalMicrosurgery
Volume30
Issue number4
DOIs
Publication statusPublished - May 2010

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Reperfusion Injury
Reperfusion
Ischemia
Venae Cavae
Erythrocytes
Cell Aggregation
Surgical Instruments
Veins
Erythrocyte Aggregation
Erythrocyte Deformability
Erythrocyte Volume
Portal Vein
Leukocyte Count
Laparotomy
Histology
Arteries

ASJC Scopus subject areas

  • Surgery

Cite this

Changes of local and systemic hemorheological properties in intestinal ischemia-reperfusion injury in the rat model. / Bráth, E.; Németh, N.; Kiss, Ferenc; Sajtos, Erika; Hever, Timea; Matyas, Lili; Tóth, L.; Mikó, I.; Furka, I.

In: Microsurgery, Vol. 30, No. 4, 05.2010, p. 321-326.

Research output: Contribution to journalArticle

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