Changes in thiol content in roots of wheat cultivars exposed to copper stress

I. Tari, G. Szalai, Z. Lôrincz, A. Bálint

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Wheat (Triticum aestivum L.) cultivars GK Tiszatáj, Yubileinaya, GK Öthalom and a landrace Kobomugi were grown for 18 d in hydroponic cultures containing 0 (control), 0.1, 1.0 or 10.0 μM Cu2+. On a dry mass basis, cvs. Tiszatáj and Kobomugi accumulated slightly more Cu2+ in the root tissues than did cvs. Yubileinaya and Öthalom, but their controls also contained higher amounts of Cu2+. As a result of perturbation in the plasma membrane functions the K+ content of roots was reduced at 10 μM Cu2+ in all cultivars, whereas the K+/Na+ ratio decreased significantly only in the roots of cv. Öthalom. In the sensitive cultivar, Öthalom, the dry mass of the roots decreased while the cysteine content, which is a limiting factor for glutathione synthesis, did not satisfactorily increase with increasing tissue Cu2+ content. This suggests that in cv. Öthalom the membrane damage of the root cells at 10 μM Cu2+ concentration may affect the sulphur availability or metabolism. Concentrations of glutathione and hydroxymethyl-glutathione, a tripeptide which may play a similar biochemical role to glutathione, were also lower in the sensitive cultivar. In the absence of glutathione the root tissues failed to cope with the oxidative stress caused by the excessive amount of Cu2+. A significant accumulation of iron in the roots of the sensitive cultivar at 10 μM Cu2+ supply enhanced the oxidative damage.

Original languageEnglish
Article number5087391
Pages (from-to)255-260
Number of pages6
JournalBiologia Plantarum
Volume45
Issue number2
DOIs
Publication statusPublished - Jan 1 2002

Keywords

  • Glutathione
  • Hydroxymethyl-glutathione
  • Iron accumulation
  • Phytochelatins
  • γ-glutamylcysteine

ASJC Scopus subject areas

  • Plant Science
  • Horticulture

Fingerprint Dive into the research topics of 'Changes in thiol content in roots of wheat cultivars exposed to copper stress'. Together they form a unique fingerprint.

  • Cite this