Changes in thiol content in roots of wheat cultivars exposed to copper stress

I. Tari, G. Szalai, Zs Lôrincz, A. Bálint

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Wheat (Triticum aestivum L.) cultivars GK Tiszatáj, Yubileinaya, GK Öthalom and a landrace Kobomugi were grown for 18 d in hydroponic cultures containing 0 (control), 0.1, 1.0 or 10.0 μM Cu2+. On a dry mass basis, cvs. Tiszatáj and Kobomugi accumulated slightly more Cu2+ in the root tissues than did cvs. Yubileinaya and Öthalom, but their controls also contained higher amounts of Cu2+. As a result of perturbation in the plasma membrane functions the K+ content of roots was reduced at 10 μM Cu2+ in all cultivars, whereas the K+/Na+ ratio decreased significantly only in the roots of cv. Öthalom. In the sensitive cultivar, Öthalom, the dry mass of the roots decreased while the cysteine content, which is a limiting factor for glutathione synthesis, did not satisfactorily increase with increasing tissue Cu2+ content. This suggests that in cv. Öthalom the membrane damage of the root cells at 10 μM Cu2+ concentration may affect the sulphur availability or metabolism. Concentrations of glutathione and hydroxymethyl-glutathione, a tripeptide which may play a similar biochemical role to glutathione, were also lower in the sensitive cultivar. In the absence of glutathione the root tissues failed to cope with the oxidative stress caused by the excessive amount of Cu2+. A significant accumulation of iron in the roots of the sensitive cultivar at 10 μM Cu2+ supply enhanced the oxidative damage.

Original languageEnglish
Pages (from-to)255-260
Number of pages6
JournalBiologia Plantarum
Volume45
Issue number2
DOIs
Publication statusPublished - 2002

Fingerprint

thiols
glutathione
copper
wheat
cultivars
tripeptides
hydroponics
landraces
cysteine
sulfur
oxidative stress
plasma membrane
Triticum aestivum
iron
metabolism
synthesis
tissues
cells

Keywords

  • γ-glutamylcysteine
  • Glutathione
  • Hydroxymethyl-glutathione
  • Iron accumulation
  • Phytochelatins

ASJC Scopus subject areas

  • Plant Science

Cite this

Changes in thiol content in roots of wheat cultivars exposed to copper stress. / Tari, I.; Szalai, G.; Lôrincz, Zs; Bálint, A.

In: Biologia Plantarum, Vol. 45, No. 2, 2002, p. 255-260.

Research output: Contribution to journalArticle

@article{34bcb765bbc04e999375338038ffd806,
title = "Changes in thiol content in roots of wheat cultivars exposed to copper stress",
abstract = "Wheat (Triticum aestivum L.) cultivars GK Tiszat{\'a}j, Yubileinaya, GK {\"O}thalom and a landrace Kobomugi were grown for 18 d in hydroponic cultures containing 0 (control), 0.1, 1.0 or 10.0 μM Cu2+. On a dry mass basis, cvs. Tiszat{\'a}j and Kobomugi accumulated slightly more Cu2+ in the root tissues than did cvs. Yubileinaya and {\"O}thalom, but their controls also contained higher amounts of Cu2+. As a result of perturbation in the plasma membrane functions the K+ content of roots was reduced at 10 μM Cu2+ in all cultivars, whereas the K+/Na+ ratio decreased significantly only in the roots of cv. {\"O}thalom. In the sensitive cultivar, {\"O}thalom, the dry mass of the roots decreased while the cysteine content, which is a limiting factor for glutathione synthesis, did not satisfactorily increase with increasing tissue Cu2+ content. This suggests that in cv. {\"O}thalom the membrane damage of the root cells at 10 μM Cu2+ concentration may affect the sulphur availability or metabolism. Concentrations of glutathione and hydroxymethyl-glutathione, a tripeptide which may play a similar biochemical role to glutathione, were also lower in the sensitive cultivar. In the absence of glutathione the root tissues failed to cope with the oxidative stress caused by the excessive amount of Cu2+. A significant accumulation of iron in the roots of the sensitive cultivar at 10 μM Cu2+ supply enhanced the oxidative damage.",
keywords = "γ-glutamylcysteine, Glutathione, Hydroxymethyl-glutathione, Iron accumulation, Phytochelatins",
author = "I. Tari and G. Szalai and Zs L{\^o}rincz and A. B{\'a}lint",
year = "2002",
doi = "10.1023/A:1015105025080",
language = "English",
volume = "45",
pages = "255--260",
journal = "Biologia Plantarum",
issn = "0006-3134",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Changes in thiol content in roots of wheat cultivars exposed to copper stress

AU - Tari, I.

AU - Szalai, G.

AU - Lôrincz, Zs

AU - Bálint, A.

PY - 2002

Y1 - 2002

N2 - Wheat (Triticum aestivum L.) cultivars GK Tiszatáj, Yubileinaya, GK Öthalom and a landrace Kobomugi were grown for 18 d in hydroponic cultures containing 0 (control), 0.1, 1.0 or 10.0 μM Cu2+. On a dry mass basis, cvs. Tiszatáj and Kobomugi accumulated slightly more Cu2+ in the root tissues than did cvs. Yubileinaya and Öthalom, but their controls also contained higher amounts of Cu2+. As a result of perturbation in the plasma membrane functions the K+ content of roots was reduced at 10 μM Cu2+ in all cultivars, whereas the K+/Na+ ratio decreased significantly only in the roots of cv. Öthalom. In the sensitive cultivar, Öthalom, the dry mass of the roots decreased while the cysteine content, which is a limiting factor for glutathione synthesis, did not satisfactorily increase with increasing tissue Cu2+ content. This suggests that in cv. Öthalom the membrane damage of the root cells at 10 μM Cu2+ concentration may affect the sulphur availability or metabolism. Concentrations of glutathione and hydroxymethyl-glutathione, a tripeptide which may play a similar biochemical role to glutathione, were also lower in the sensitive cultivar. In the absence of glutathione the root tissues failed to cope with the oxidative stress caused by the excessive amount of Cu2+. A significant accumulation of iron in the roots of the sensitive cultivar at 10 μM Cu2+ supply enhanced the oxidative damage.

AB - Wheat (Triticum aestivum L.) cultivars GK Tiszatáj, Yubileinaya, GK Öthalom and a landrace Kobomugi were grown for 18 d in hydroponic cultures containing 0 (control), 0.1, 1.0 or 10.0 μM Cu2+. On a dry mass basis, cvs. Tiszatáj and Kobomugi accumulated slightly more Cu2+ in the root tissues than did cvs. Yubileinaya and Öthalom, but their controls also contained higher amounts of Cu2+. As a result of perturbation in the plasma membrane functions the K+ content of roots was reduced at 10 μM Cu2+ in all cultivars, whereas the K+/Na+ ratio decreased significantly only in the roots of cv. Öthalom. In the sensitive cultivar, Öthalom, the dry mass of the roots decreased while the cysteine content, which is a limiting factor for glutathione synthesis, did not satisfactorily increase with increasing tissue Cu2+ content. This suggests that in cv. Öthalom the membrane damage of the root cells at 10 μM Cu2+ concentration may affect the sulphur availability or metabolism. Concentrations of glutathione and hydroxymethyl-glutathione, a tripeptide which may play a similar biochemical role to glutathione, were also lower in the sensitive cultivar. In the absence of glutathione the root tissues failed to cope with the oxidative stress caused by the excessive amount of Cu2+. A significant accumulation of iron in the roots of the sensitive cultivar at 10 μM Cu2+ supply enhanced the oxidative damage.

KW - γ-glutamylcysteine

KW - Glutathione

KW - Hydroxymethyl-glutathione

KW - Iron accumulation

KW - Phytochelatins

UR - http://www.scopus.com/inward/record.url?scp=0036209133&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036209133&partnerID=8YFLogxK

U2 - 10.1023/A:1015105025080

DO - 10.1023/A:1015105025080

M3 - Article

AN - SCOPUS:0036209133

VL - 45

SP - 255

EP - 260

JO - Biologia Plantarum

JF - Biologia Plantarum

SN - 0006-3134

IS - 2

ER -