Changes in the expression of differentiated functions during long-term cultivation of rat hepatoma cells

Anikó Venetianer, Zsuzsanna Bösze

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The stability of the expression of six differentiated functions was examined during long-term cultivation of rat hepatoma cells. Faza 967 cell line-a clonal descendant of the Reuber H35 hepatoma-is characterized by the activity of tyrosine aminotransferase (TAT) and gluconeogenetic enzymes; secretion of serum albumin; and the presence of liver isozymes of alcohol dehydrogenase (ADH-L), aldolase (aldolase-B) and five isozymes of lactate dehydrogenase (LDH). During the 3-year-long cultivation of Faza 967 cells TAT specific activity, inducibility, and albumin production were reduced drastically whereas the expression of the three liver-specific isozymes examined was maintained. The majority of Faza 967 cells were able to perform gluconeogenesis after 3 years of continuous cultivation. Our results show that long-term cultivation of hepatoma cells may change the expression of certain liver-specific functions independently of the expression of other differentiated functions.

Original languageEnglish
Pages (from-to)85-93
Number of pages9
JournalSomatic Cell Genetics
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 1 1983

ASJC Scopus subject areas

  • Genetics

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