Changes in the expression and distribution of the inducible and endothelial nitric oxide synthase in mucosal biopsy specimens of inflammatory bowel disease

K. Palatka, Z. Serfőző, Zoltán Veréb, Zoltán Hargitay, Bea Lontay, F. Erdődi, G. Bánfalvi, Z. Nemes, M. Udvardy, I. Altorjay

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Abstract

Objective. The role of nitric oxide (NO) in the pathophysiology of inflammatory bowel disease (IBD) is controversial. The aim of this study was to investigate the expression and localization of nitric oxide synthase isoforms (iNOS, eNOS) in IBD colonic mucosa. Material and methods. Forty-four patients with IBD (24 ulcerative colitis (UC), 20 Crohn's disease (CD) and 16 controls) were investigated by colonoscopy. iNOS and eNOS in tissue sections was demonstrated by histochemistry (NADPH-diaphorase reaction) and immunohistochemistry. Cell type analysis and quantitative assessment of the iNOS immunoreactive (IR) cells and densitometry of iNOS in immunoblots were also performed. Results. iNOS-IR cells were significantly numerous in inflamed mucosa of UC (64 ± 4 cells/mm2) than in CD (4 ± 2 cells/mm2). iNOS-IR/ CD15-IR cells showed significant elevation in inflamed (i) versus uninflamed (u) UC mucosa (UCu 8 ± 3%, UCi 85 ± 10%) In CD, the percentage of iNOS-IR/CD68-IR cells was lower in inflamed sites (CDu 23 ± 8%, CDi 4 ± 3%). Immunoblot of biopsies revealed significant elevation of iNOS in active UC compared with uninflamed sites, whereas in CD no significant changes were detected. Differences were observed in eNOS and endothelial marker CD31 immunoreactivity. In patients with UC and in controls the ratios of eNOS/CD31-IR vessels were 82.3% and 92.0% respectively, whereas in CD the ratio was 8.3% with a concomitantly significant increase of CD31-IR vessels. The distribution and morphological characteristics of the NOS-IR inflammatory cells and endothelia were similar to those showing NADPH-diaphorase reactivity. Conclusions. Differences observed in the expression and distribution of NOS isoforms in immune and endothelial cells may contribute to better understanding of the structural and physiological changes in UC and CD.

Original languageEnglish
Pages (from-to)670-680
Number of pages11
JournalScandinavian Journal of Gastroenterology
Volume40
Issue number6
DOIs
Publication statusPublished - Jun 2005

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Nitric Oxide Synthase Type III
Nitric Oxide Synthase Type II
Inflammatory Bowel Diseases
Ulcerative Colitis
Crohn Disease
Biopsy
NADPH Dehydrogenase
Mucous Membrane
Protein Isoforms
Densitometry
Colonoscopy
Nitric Oxide Synthase
Endothelium
Nitric Oxide
Endothelial Cells
Immunohistochemistry

Keywords

  • Crohn's disease
  • Histopathology
  • Inflammatory bowel disease
  • Nitric oxide synthase
  • Ulcerative colitis

ASJC Scopus subject areas

  • Gastroenterology

Cite this

@article{6aa8908d17c24b508443f798bd1f43d7,
title = "Changes in the expression and distribution of the inducible and endothelial nitric oxide synthase in mucosal biopsy specimens of inflammatory bowel disease",
abstract = "Objective. The role of nitric oxide (NO) in the pathophysiology of inflammatory bowel disease (IBD) is controversial. The aim of this study was to investigate the expression and localization of nitric oxide synthase isoforms (iNOS, eNOS) in IBD colonic mucosa. Material and methods. Forty-four patients with IBD (24 ulcerative colitis (UC), 20 Crohn's disease (CD) and 16 controls) were investigated by colonoscopy. iNOS and eNOS in tissue sections was demonstrated by histochemistry (NADPH-diaphorase reaction) and immunohistochemistry. Cell type analysis and quantitative assessment of the iNOS immunoreactive (IR) cells and densitometry of iNOS in immunoblots were also performed. Results. iNOS-IR cells were significantly numerous in inflamed mucosa of UC (64 ± 4 cells/mm2) than in CD (4 ± 2 cells/mm2). iNOS-IR/ CD15-IR cells showed significant elevation in inflamed (i) versus uninflamed (u) UC mucosa (UCu 8 ± 3{\%}, UCi 85 ± 10{\%}) In CD, the percentage of iNOS-IR/CD68-IR cells was lower in inflamed sites (CDu 23 ± 8{\%}, CDi 4 ± 3{\%}). Immunoblot of biopsies revealed significant elevation of iNOS in active UC compared with uninflamed sites, whereas in CD no significant changes were detected. Differences were observed in eNOS and endothelial marker CD31 immunoreactivity. In patients with UC and in controls the ratios of eNOS/CD31-IR vessels were 82.3{\%} and 92.0{\%} respectively, whereas in CD the ratio was 8.3{\%} with a concomitantly significant increase of CD31-IR vessels. The distribution and morphological characteristics of the NOS-IR inflammatory cells and endothelia were similar to those showing NADPH-diaphorase reactivity. Conclusions. Differences observed in the expression and distribution of NOS isoforms in immune and endothelial cells may contribute to better understanding of the structural and physiological changes in UC and CD.",
keywords = "Crohn's disease, Histopathology, Inflammatory bowel disease, Nitric oxide synthase, Ulcerative colitis",
author = "K. Palatka and Z. Serfőző and Zolt{\'a}n Ver{\'e}b and Zolt{\'a}n Hargitay and Bea Lontay and F. Erdődi and G. B{\'a}nfalvi and Z. Nemes and M. Udvardy and I. Altorjay",
year = "2005",
month = "6",
doi = "10.1080/00365520510015539",
language = "English",
volume = "40",
pages = "670--680",
journal = "Scandinavian Journal of Gastroenterology",
issn = "0036-5521",
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TY - JOUR

T1 - Changes in the expression and distribution of the inducible and endothelial nitric oxide synthase in mucosal biopsy specimens of inflammatory bowel disease

AU - Palatka, K.

AU - Serfőző, Z.

AU - Veréb, Zoltán

AU - Hargitay, Zoltán

AU - Lontay, Bea

AU - Erdődi, F.

AU - Bánfalvi, G.

AU - Nemes, Z.

AU - Udvardy, M.

AU - Altorjay, I.

PY - 2005/6

Y1 - 2005/6

N2 - Objective. The role of nitric oxide (NO) in the pathophysiology of inflammatory bowel disease (IBD) is controversial. The aim of this study was to investigate the expression and localization of nitric oxide synthase isoforms (iNOS, eNOS) in IBD colonic mucosa. Material and methods. Forty-four patients with IBD (24 ulcerative colitis (UC), 20 Crohn's disease (CD) and 16 controls) were investigated by colonoscopy. iNOS and eNOS in tissue sections was demonstrated by histochemistry (NADPH-diaphorase reaction) and immunohistochemistry. Cell type analysis and quantitative assessment of the iNOS immunoreactive (IR) cells and densitometry of iNOS in immunoblots were also performed. Results. iNOS-IR cells were significantly numerous in inflamed mucosa of UC (64 ± 4 cells/mm2) than in CD (4 ± 2 cells/mm2). iNOS-IR/ CD15-IR cells showed significant elevation in inflamed (i) versus uninflamed (u) UC mucosa (UCu 8 ± 3%, UCi 85 ± 10%) In CD, the percentage of iNOS-IR/CD68-IR cells was lower in inflamed sites (CDu 23 ± 8%, CDi 4 ± 3%). Immunoblot of biopsies revealed significant elevation of iNOS in active UC compared with uninflamed sites, whereas in CD no significant changes were detected. Differences were observed in eNOS and endothelial marker CD31 immunoreactivity. In patients with UC and in controls the ratios of eNOS/CD31-IR vessels were 82.3% and 92.0% respectively, whereas in CD the ratio was 8.3% with a concomitantly significant increase of CD31-IR vessels. The distribution and morphological characteristics of the NOS-IR inflammatory cells and endothelia were similar to those showing NADPH-diaphorase reactivity. Conclusions. Differences observed in the expression and distribution of NOS isoforms in immune and endothelial cells may contribute to better understanding of the structural and physiological changes in UC and CD.

AB - Objective. The role of nitric oxide (NO) in the pathophysiology of inflammatory bowel disease (IBD) is controversial. The aim of this study was to investigate the expression and localization of nitric oxide synthase isoforms (iNOS, eNOS) in IBD colonic mucosa. Material and methods. Forty-four patients with IBD (24 ulcerative colitis (UC), 20 Crohn's disease (CD) and 16 controls) were investigated by colonoscopy. iNOS and eNOS in tissue sections was demonstrated by histochemistry (NADPH-diaphorase reaction) and immunohistochemistry. Cell type analysis and quantitative assessment of the iNOS immunoreactive (IR) cells and densitometry of iNOS in immunoblots were also performed. Results. iNOS-IR cells were significantly numerous in inflamed mucosa of UC (64 ± 4 cells/mm2) than in CD (4 ± 2 cells/mm2). iNOS-IR/ CD15-IR cells showed significant elevation in inflamed (i) versus uninflamed (u) UC mucosa (UCu 8 ± 3%, UCi 85 ± 10%) In CD, the percentage of iNOS-IR/CD68-IR cells was lower in inflamed sites (CDu 23 ± 8%, CDi 4 ± 3%). Immunoblot of biopsies revealed significant elevation of iNOS in active UC compared with uninflamed sites, whereas in CD no significant changes were detected. Differences were observed in eNOS and endothelial marker CD31 immunoreactivity. In patients with UC and in controls the ratios of eNOS/CD31-IR vessels were 82.3% and 92.0% respectively, whereas in CD the ratio was 8.3% with a concomitantly significant increase of CD31-IR vessels. The distribution and morphological characteristics of the NOS-IR inflammatory cells and endothelia were similar to those showing NADPH-diaphorase reactivity. Conclusions. Differences observed in the expression and distribution of NOS isoforms in immune and endothelial cells may contribute to better understanding of the structural and physiological changes in UC and CD.

KW - Crohn's disease

KW - Histopathology

KW - Inflammatory bowel disease

KW - Nitric oxide synthase

KW - Ulcerative colitis

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DO - 10.1080/00365520510015539

M3 - Article

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EP - 680

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JF - Scandinavian Journal of Gastroenterology

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