Changes in the Distribution of Type II Transmembrane Serine Protease, TMPRSS2 and in Paracellular Permeability in IPEC-J2 Cells Exposed to Oxidative Stress

Erzsebet Paszti-Gere, Reka Fanni Barna, Csaba Kovago, Ipoly Szauder, Gabriella Ujhelyi, Csaba Jakab, Nóra Meggyesházi, A. Székács

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The effect of oxidative stress on barrier integrity and localization of transmembrane serine proteinase 2 (TMPRSS2) were studied using porcine epithelial IPEC-J2 cells on membrane inserts. Increased paracellular permeability of FITC-dextran 4 kDa (fluorescence intensity 43,508 ± 2,391 versus 3,550 ± 759) and that of gentamicin (3.41 ± 0.06 % increase to controls) were measured parallel with the reduced transepithelial electrical resistance (23.3 ± 4.06 % decrease) of cell layers 6 h after 1 h 1 mM H2O2 treatment. The immunohistochemical localization of adherens junctional β-catenin was not affected by reactive oxygen species (ROS) up to 4 mM H2O2. Peroxide-triggered enhanced paracellular permeability of IPEC-J2 cell layer was accompanied by predominantly cytoplasmic occurrence of TMPRSS2 embedded in cell membrane under physiological conditions. These results support that ROS can influence paracellular gate opening via multifaceted mode of action without involvement of β-catenin redistribution in adherens junction. Altered distribution pattern of TMPRSS2 and relocalized transmembrane serine protease activity may contribute to weakening of epithelial barrier integrity under acute oxidative stress.

Original languageEnglish
Pages (from-to)775-783
Number of pages9
JournalInflammation
Volume38
Issue number2
DOIs
Publication statusPublished - 2015

Fingerprint

Catenins
Serine Proteases
Permeability
Reactive Oxygen Species
Oxidative Stress
Cell Membrane
Adherens Junctions
Peroxides
Gentamicins
Electric Impedance
Swine
Fluorescence
fluorescein isothiocyanate dextran

Keywords

  • IPEC-J2
  • oxidative stress
  • paracellular permeability
  • TMPRSS2
  • β-catenin

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Changes in the Distribution of Type II Transmembrane Serine Protease, TMPRSS2 and in Paracellular Permeability in IPEC-J2 Cells Exposed to Oxidative Stress. / Paszti-Gere, Erzsebet; Barna, Reka Fanni; Kovago, Csaba; Szauder, Ipoly; Ujhelyi, Gabriella; Jakab, Csaba; Meggyesházi, Nóra; Székács, A.

In: Inflammation, Vol. 38, No. 2, 2015, p. 775-783.

Research output: Contribution to journalArticle

Paszti-Gere, Erzsebet ; Barna, Reka Fanni ; Kovago, Csaba ; Szauder, Ipoly ; Ujhelyi, Gabriella ; Jakab, Csaba ; Meggyesházi, Nóra ; Székács, A. / Changes in the Distribution of Type II Transmembrane Serine Protease, TMPRSS2 and in Paracellular Permeability in IPEC-J2 Cells Exposed to Oxidative Stress. In: Inflammation. 2015 ; Vol. 38, No. 2. pp. 775-783.
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